Cereal Chem 65:153-154   |  VIEW ARTICLE

Note: Rapid Electrophoresis of Oat (Avena sativa L.) Prolamins from Single Seeds for Cultivar Identification.

A. E. Hansen, A. Nassuth, and I. Altosaar. Copyright 1988 by the American Association of Cereal Chemists, Inc. 

A quick method for the extraction of alcohol-soluble proteins (prolamins) of oats from single seeds and flour samples was developed. Sodium dodecyl sulfate polyacrylamide gel electrophoresis was carried out using a programmable, microporcessor-controlled electrophoresis unit and ultrathin prefabricated polyacrylamide gels. This system allowed gels to be run, stained, and destained in 1 hr. The prolamin patterns from seven different oat cultivars were dufficiently unique for cultivar identification. With this equipment, most variables that hindered reproducibility in the past were removed. With minimal experience it was possible to obtain standardized, repeatable electrophoregram prolamin patterns quickly.