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Cereal Chem 37:93 - 96.  |  VIEW ARTICLE

Determination of Uric Acid in Wheat Flour Infested by Tribolium castaneum Duv., Using Paper Chromatography

S. Venkatrao, K. Krishnamurthy, M. Swaminathan, and V. Subrahmanyan. Copyright 1960 by the American Association of Cereal Chemists, Inc. 

The uric acid present in infested wheat flour was separated by paper chromatography and quantitatively estimated using Benedict's uric acid reagent. The results agreed with those obtained by a direct colorimetric method of determining uric acid in protein-free aqueous extracts of infested flour. The uric acid content in the infested flour increased steadily with the progress of infestation. No uric acid could be detected in the extract of control uninfested flour. The protein-free aqueous extract of control uninfested wheat flour, however, contained small amounts of substances (other than uric acid) which reacted with the Benedict's uric acid reagent and yielded an "apparent" uric acid value. This value was low, as compared with those found in the infested flour.

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