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Cereal Chem 40:337 - 342.  |  VIEW ARTICLE

Silicic Acid Chromatography of Wheat Lipids.

J. H. Nelson, R. L. Glass, and W. F. Geddes. Copyright 1963 by the American Association of Cereal Chemists, Inc. 

Lipid material extracted from whole-wheat meal by water-saturated l-butanol was chromatographed on silicic acid columns. With a gradient elution so1vent system of increasing concentrations of ethyl ether in petroleum ether followed by absolute methanol, wheat lipids were separated reproducibly into four fractions of increasing polarity. Fraction I presumably contained hydrocarbons and steryl esters; fraction II consisted almost entirely of triglycerides; fraction III was very heterogeneous and contained, among other compounds, the bulk of the wheat pigments; and fraction IV, which contained phosphorus, nitrogen, and sugars, was presumed to consist largely of phospholipids and glycolipids. Wheat lipids extractable by water-saturated l-butanol but not by ethyl ether consist almost entirely of polar material as distinguished from the predominantly nonpolar nature of the ethyl ether-extractable substances. Marked differences in the lipids of bran, germ, and "endosperm" were observed.

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