Cereal Chem 51:112 - 117. | VIEW
Studies of Glutenin. V. Note on Additional Preparative Methods.
R. J. Wasik and W. Bushuk. Copyright 1974 by the American Association of Cereal Chemists, Inc.
Three methods, namely, gel filtration chromatography, pH precipitation with purification on SE Sephadex C-50, and ammonium sulfate precipitation, were compared for preparing glutenins from durum wheat. Polyacrylamide gel electrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and amino acid analyses showed that the third method, which is considerably easier to perform, produces relatively pure glutenin.