Cereal Chem 56:227 - 231.  |  VIEW ARTICLE
Molecular-Weight Distribution of Soybean Globulin Peptides Produced by Peptic Hydrolysis.

D. S. T. Hsieh, C. Lin, E. R. Lang, N. Catsimpoolas, and C. K. Rha. Copyright 1979 by the American Association of Cereal Chemists, Inc. 

Soybean globulin dispersions (5% w/v) were hydrolyzed with pepsin (enzyme/substrate = 1/100, 2/100) at pH 1.6 at 25 C for various time intervals. Molecular-weight distribution analysis of the buffer-soluble peptides was performed by gel filtration chromotography using a standardized Sephadex G-50 column. There was a rapid decrease in weight-average molecular weight within the first few minutes of hydrolysis. The logarithm of the weight-average molecular weight of the peptides and the hydrolysis time (10 to 70 min) had an approximately linear relationship. This empirical observation can be used to predict the peptic hydrolysis time needed to obtain soluble soybean protein peptides of a desired molecular size.