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Cereal Chem 58:467 - 470.  |  VIEW ARTICLE
Starch Determination in Some Dietary Fiber Sources.

F. R. Dintzis and C. C. Harris. Copyright 1981 by the American Association of Cereal Chemists, Inc. 

Commercially available and relatively inexpensive enzymes, single glucoamylase preparations and a high- temperature, dual system of alpha-amylase plus glucoamylase, were used successfully to determine starch in dietary fiber sources such as some cereal brans that contain large amounts of cellulose and hemicellulose. Gas chromatography was used to measure neutral sugars released by the enzyme preparations, glucose oxidase-peroxidase to measure released glucose only, and optical rotation to directly measure starch content in CaCl2 or dimethyl sulfoxide solution. Incubation of autoclaved substrate with glucoamylase at 50 C for 2 hr or use of high temperature alpha-amylase (75 C for 20 min) plus glucoamylase (65 C for 35 min) appeared to minimize the effects of suspected hemicellulase and cellulase impurities. Both enzyme systems and methods of detecting glucose yielded similar starch contents of bran materials. Starch values obtained by optical rotation for wheat brans were lower than those measured by enzyme methods.

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