Cereal Chem 61:205 - 208. | VIEW
Rapid Analysis of Changes in the Molecular Weight Distribution of Buffer-soluble Proteins During Germination of Wheat.
J. E. Kruger. Copyright 1984 by the American Association of Cereal Chemists, Inc.
A high-performance liquid chromatography (HPLC) system equipped with Spherogel-TSK 3000 aqueous size-exclusion column and a computing integrator with a molecular weight program was used to monitor changes in buffer-soluble wheat protein components during germination of wheat kernels. At least seven major components were present. During germination, small molecular weight entities were progressively formed from proteins; some bind to the column and have longer column-elution times than their molecular weights would indicate. Such changes can indicate the approximate degree of germination of wheat kernels. Molecular weight profiles of extracts changed upon standing, indicating the presence of proteolytic enzymes. Cultivar differences in chromatographic elution profiles were very small.