Cereal Chem. 71:409-411 | VIEW
Antigenic Properties of Albumin, Globulin, and Protein Concentrate Fractions from Rice Bran.
P. S. Landers and B. R. Hamaker. Copyright 1994 by the American Association of Cereal Chemists, Inc.
Alkali-extracted rice bran protein was tested for antigenic properties by development of polyclonal antibodies in the rabbit. Animals were also inoculated with soy protein isolate and nonfat dry milk. Titers, determined by enzyme-linked immunosorbent assay, for the three groups were compared. Soy protein isolate evoked the highest class G immunoglobulin (IgG) antibody titer (21,845,000), followed by rice bran protein (655,000), and nonfat dry milk (27,000). Rice bran proteins consisted of 29% albumins, 23% globulins, and 20% glutelins. Albumins and globulins made up 69% of the extractable protein. Albumins, globulins, and two rice bran protein concentrates, produced by extraction in alkali followed by isoelectric precipitation or spray-drying, were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and reacted against the antibran protein polyclonal antibodies in Western blot analysis. A small amount of highly antigenic protein (approximately 16 kDa) was found in the alkali-extracted rice bran protein concentrates, but it was absent in the albumin and globulin protein fractions.