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Cereal Chem. 71:469-471   |  VIEW ARTICLE

Rapid Method for Estimation of Amylose in Maize Starches.

C. A. Knutson and M. J. Grove. Copyright 1994 by the American Association of Cereal Chemists, Inc. 

A rapid solubilization procedure for maize starch, using low- temperature gelatinization in 3M CaCl2 in combination with 15-30 min of sonication in an iodine-dimethyl sulfoxide solution at a temperature of 65- 70 C makes it possible to estimate amylose content of maize samples in less than 1 hr. Results obtained by this technique are equivalent to those obtained by conventional thermal dissolution of starch in iodine- dimethyl sulfoxide, which requires overnight heating to obtain complete solubilization.

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