Cereal Chem 72:598-601 |
Relationships Between Polymeric Glutenin and the Quality Characteristics for Seven Common Wheats (Triticum aestivum) Grown in the Field and Greenhouse.
L. El Haddad, T. Aussenac, J.- L. Fabre, and A. Sarrafi. Copyright 1995 by the American Association of Cereal Chemists, Inc.
Seven common wheat cultivars were grown under greenhouse and field conditions. Sodium dodecyl sulfate (SDS) extraction and size exclusion high-performance liquid chromatography (SE-HPLC) were used to separate proteins according to size (pF1-pF5 and pFi). The technological parameters such as alveograph index (W, P, G) and Zeleny volume (Z) of each genotype for each agronomic condition were measured. A high genetic variability was observed for all parameters. Differences between the two agronomic conditions were significant for most parameters, however, the interactions between genotype and agronomic conditions were significant only for a few protein fractions. The amount of certain polymeric glutenin classifies the genotypes in the same way as quality criteria (Zeleny and alveograph) do. The ratio of the amount of soluble polymeric glutenin excluded by column of SE- HPLC (pF1) to the amount of non-excluded ones (pF2) divides genotypes into two groups, one with HMW-glutenin subunits 5+10 and the other with HMW- glutenin subunits 2+12 (Glu-D1 allelic composition).