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Cereal Chem. 73 (5):547-550  |  VIEW ARTICLE

Analytical Techniques and Instrumentation

Identification by High-Performance Capillary Electrophoresis of Wheat Lines Containing the 1AL.1RS and the 1BL.1RS Translocation (1).

G. L. Lookhart (2), S. R. Bean (3), R. Graybosch (4), O.K. Chung (2), B. Morena-Sevilla (5), and S. Baenziger (5). (1) Contribution of the USDA-ARS and the Department of Grain Science and Industry, Kansas State University. Journal series no. 96-253-J. Mention of firm names or trade products does not constitute endorsement by the U.S. Department of Agriculture. (2) Research chemist, USDA-ARS, U.S. Grain Marketing Research Laboratory, Manhattan, KS 66502. Corresponding author. E-mail: <george@usgmrl.ksu.edu> (3) Research assistant, Grain Science and Industry, Kansas State University, Manhattan, KS 66506. (4) Research geneticist, USDA-ARS, Wheat, Sorghum and Forage Research Unit, University of Nebraska, Lincoln, NE 68583. (5) Research assistant and professor, respectively, Department of Agronomy, University of Nebraska, Lincoln, NE 68583. Accepted June 12, 1996. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. American Association of Cereal Chemists, Inc., 1996. 

High-performance capillary electrophoresis (HPCE) was used to analyze grain proteins from U.S. wheat cultivars carrying 1AL.1RS from Amigo or 1BL.1RS from Kavkaz wheat-rye chromosomal translocations. To date, these are the only 1RS translocations known to occur in U.S. cultivars. Chromosome specific patterns were observed among released cultivars and experimental lines. The method was verified by analyzing protein extracts of the heterogeneous cultivars Nekota and Rawhide and their homogeneous, homozygous non-1RS progeny. The purified 1AL.1RS and 1BL.1RS progenies were derived from Nekota and Rawhide, respectively. HPCE provides a rapid and efficient method for detection of flour or grain derived from 1AL.1RS and 1BL.1RS wheats, and can differentiate the two types of translocations.

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