Sunset Yellow: A Food Color for Protein Staining with SDS-PAGE
M. Badaruddin (1), S. U. Abdullah (1), S. A. Sayeed (2), R. Ali (1), and M. N. Riaz (3). (1) Division of Food Research, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi. (2) National Foods Ltd., S.I.T.E, Karachi, Pakistan. (3) Food Protein R&D Center, Texas A&M University, College Station, TX 77843-2476. Cereal Foods World 52(1)12-14.
This study shows that Sunset Yellow FCF is a fast-reacting dye for food proteins and may be used as a fast staining agent for all types of resolved proteins with PAGE, which is important because Coomassie Brilliant Blue R 250 and Amido Black 10 B take at least 24 hr for visualization of proteins bands with PAGE. Sunset Yellow FCF is highly sensitive, rapid, lasting, and provides reproducible results with a variety of proteins. It produces sharp, nondiffuseable, clear, distinct, and stable bands on gel. It requires just 1 hr for staining and destaining to visualize the known proteins separated on the acrylamide gels.