Detecting and Quantifying GMOs in Grain Using Protein Methods
V. Pantella. VIP Consulting Services, Silver Spring, MD. Cereal Foods World 52(4):189-190.
A number of fundamental aspects must be considered when applying immunoassays (protein-based methodology) to the detection of genetically modified organisms (GMOs) in grain. Many factors such as genetics and the environment, processing, and biological and statistical errors affect the end result. The most commonly used methodology includes lateral flow devices and enzyme-linked immunosorbent assays. The need for regulations which consider the critical aspects involved in the accurate detection of GMOs is a necessity to ensure reliable results across different laboratories and to create stability in the global marketplace. Recently, a consortium of experts dedicated to the goal of achieving analytical excellence in the field of biotechnology published a manuscript in the Journal of AOAC International that details the use of protein-based genetically modified organism test methods for the agricultural biotechnology industry. The following article provides an introduction to the topic and a summary of some of the salient issues.