Suppliers Guide​

Companies are listed here for the convenience of users of the AACC Approved Methods of Analysis, 11th Edition. Specific equipment (Apparatus) or chemicals listed in the method should not be substituted without confirmation of equivalent method performance. Suppliers for general laboratory equipment and chemicals are not recommended over suppliers of equivalent products.


02-01.02, Fat Acidity - General Method

Thimbles. Alundum AN 899 thimbles, series 09-660, Fisher Scientific


02-02.02, Fat Acidity - Rapid Method for Samll Grains

Grinder-extractor, Stein mil, model M-2, or equivalent, Steinlite Corp.


04-22.01, Citric and Isocitric Acids

Silcic acid suitable for chromatography, anaytical reagent, 100-mesh (No. 2847 Mallinckrodt Baker, or equivalent). Mallincrodt Laboratory Chemicals, a division of Mallinckrodt baker Inc.


04-27.01, Tartaric Acid-Quantitative Method

Ceramic Fiber


07-01.01, Measurement of Acid-Stable Amino Acids

Hydrolysis tubes, reusable vacuum Hydrolysis tubes, and amino acid standards. Pierce Protein Research Products, a part of Thermo Fisher Scientific


07-11.01, Measurement of Sulfur Amino Acids

Cysteic acid (Sigma, Cat. no. C-7630) and L-Methionine sulfone (Sigma, Cat. no. M-0876). Sigma Chemical Co.

Buffer preservative (pHix). Pierce Protein Research Products, a part of Thermo Fisher Scientific


07-20.01, Measurement of Tryptophan-Alkaaline Hydrolysis

HPLC. Waters Associates 6000 model (cap) or equivalent, with 25-cm x 4.6-mm inside diameter stainless-steel column packed with C18 m-bondapak and equipped with 280-nm detector. Waters Associates
Hydrolysis tubes, reusable vacuum Hydrolysis tubes (Cat. nos. 29550, 29560, 29562, 29564), Buffer preservative (pHix), ninhydrin, and sodium citrate loading buffer. Pierce Protein Research Products, a part of Thermo Fisher Scientific


08-11.01, Ash in Dry Milk Products

Procelain crucible. Sigma Chemical Co.


08-12.01, Ash in Farina and Semolina

Procelain crucible. Sigma Chemical Co.


08-16.01, Ash in Soy Flour

Porcelain combustion capsules, Coors no. 60050, outside diam. 69-mm x height 12-ml, 250ml capacity. CoorsTek


08-21.01, Prediction of Ash Content in Wheat Flour-Near-Infrared Method

Near-infared instrument. Inframatic 86 Series Ash and Inframatic 9140, Perten Instruments, Inc.


10-09.01, Basic Straight-Dough Bread-Baking Method-Long Fermentation

Various equipment (mixers, fermentation cabinet, sheeter/molder, loaf volunteer).National Manufacturing Co., a division of TMCO, Inc.
Baking pans. American Pan Co.
Oven. Reed Oven Co.


10-10.03, Optimized Straight-Dough Bread-Making Method

Mixers for doughs for 1-lb loaves. Hobart HL120 or HL200 electric mixer. Hobart Corp
Various Pieces apparatus, including pin-type mixers for 10-and 100g flour doughs. National Manufacturing Co., a division of TMCO, Inc.


10-11.01, Baking Quaity of Bread Flour-Sponge-Dough, Pound-Loaf Method

Mixer, Hobart HL120 or HL200 electric mixer. Hobart Corp
Bowl, McDuffee-type, equipped with water jacket and mixing fork. National Manufacturing Co.,, a division of TMCO, Inc.
Various equipment (mixers, fermentation cabinet, sheeter/molder, loaf volumeter). National Manufacturing Co.,, a division of TMCO, Inc.
Baking Pans American Pan Co.
Oven Reed Oven Co.


10-13.02, Guidelines for Testing a Variety of Products

Mixer. Hobart Corp.

Bowl, McDuffee type. National Manufacturing Co.
Tortilla press. DOUGHPRO Co.
Spiral mixer for bagels. Model XTS 20C, Excalibur Bagel and Bakery Equipment, Inc.,
Bagel former. Model CG/DF 4000, G. CINELLI ESPERIA, Corp.


10-14.01, Determination of Bread Volume by Laser Topography: BVM Method

BVM bread volume meter. Model 6610 (part no. 66191), model 6630 (part no. 66451). Perten Instruments


10-15.01, Baking Quality of Angel-Cake Flour

Electric mixer. Such as Hobart C-100, with 10-qt (9.5-liter) bowl and nominal orbital speeds (rpm) of low = 61, medium = 109, high = 190, or Hobart HL120, with 12-qt (11.4 liter) bowl and nominal orbital speeds (rpm) of low = 61, medium = 113, high = 205. Hobart Corp.


10-16.01, Volumetric and Dimensional Profile of Baked Products by Laser Topography — VolScan Profiler Method

VolScan Profiler, model VSP300 or VSP600. Stable Micro Systems
Texture Technologies


10-18.01, Measurement of Crumb Structure of Baked Products by C-Cell

C-Cell, monochrome system (CC.300) or color system (CC.400) with serial numbers above 205030. Calibration card, CC001 (monochrome system) or CC002 (color system). Calibre Control


10-31.03, Baking Quality of Biscuit Flour

Electric mixer. Such as Hobart C-100 with 3-qt (2.8 liter) bowl and nominal orbital speeds (rpm) of low = 61, medium = 109, high = 190, or Hobart N50 with 5-qt (4.7-liter) bowl and nominal orbital speeds (rpm) of low = 61, medium = 125, high = 259, Hobart Corp., or KitchenAid KSM 150PSOB, with 5-qt (4.7-liter) bowl and nominal orbital speeds (rpm) of stir speed = 61, speed no. 2 = 95, speed no. 6 = 180, KitchenAid
Cookie sheets, gauge strips, and cutter. Wayne Machine Shop, Inc.
Shortening. Such as Centrasoy "G". Essex Grain Products


10-50.05, Baking Quality of Cookie Flour

Electric mixer. Such as Hobart C-100, with 3-qt (2.8-liter) bowl or 5-qt (4.7-liter) bowl and nominal orbital speeds (rpm) of low = 61, medium = 109, high = 190, or Hobart N50 with 5-qt (4.7-liter) bowl and nominal orbital speeds (rpm) of low = 61, medium = 125, high = 259, Hobart Corp., or KitchenAid KSM 150PSOB, with 5-qt (4.7-liter) bowl and nominal orbital speeds (rpm) of stir speed = 61, speed no. 2 = 95, speed no. 6 = 180, KitchenAid
Cookie sheets, gauge strips, and cutter, Wayne Machine Shop, Inc.
Refractory sheet. ZIRCAR Refractory Composites, Inc.


10-52.02, Baking Quality of Cookie Flour-Micro Method

National cookie dough micromixer and special cookie dough bowl. National manufacturing Co., a division of TMCO.
electric mixer for creamed mass. Such as Hobart C-100 with 10-qt (9.5-liter) bowl and nominal orbital speeds (rpm) of low = 61, medium = 109, high = 190, or Hobart HL120, with 12-qt (11.4 liter) bowl and nominal orbital speeds (rpm) of low = 61, medium = 113, high = 205, Hobart Corp., or KitchenAid KSM 150PSOB, with 5-qt (4.7-liter) bowl and nominal orbital speeds (rpm) of stir speed = 61, speed no. 2 = 95, speed no. 6 = 180, KitchenAid
Cookie sheets, gauge strips, and cutter. Wayne Machine Shop, Inc.
Refractory sheet. ZIRCAR Refractory Composites, Inc.


10-53.01, Baking Quality of Cookie Flour-Macro Wire-Cut Formulation

Electric mixer. Such as Hobart C-100, with 3-qt (2.8-liter) bowl or 5-qt (4.7-liter) bowl and nominal orbital speeds (rpm) of low = 61, medium = 109, high = 190, or Hobart N50 with 5-qt (4.7-liter) bowl and nominal orbital speeds (rpm) of low = 61, medium = 125, high = 259, Hobart Corp., or KitchenAid KSM 150PSOB, with 5-qt (4.7-liter) bowl and nominal orbital speeds (rpm) of stir speed = 61, speed no. 2 = 95, speed no. 6 = 180, KitchenAid
Cookie sheets, gauge strips, and cutter. Wayne Machine Shop, Inc.
Refractory sheet. ZIRCAR Refractory Composites, Inc.


​10-54.01​​, Baking Quality of Cookie Flour-Micro Wire-Cut Formulation

Electric mixer for creamed mass. Such as Hobart C-100 with 10-qt (9.5-liter) bowl and nominal orbital speeds (rpm) of low = 61, medium = 109, high = 190, or Hobart HL120, with 12-qt (11.4 liter) bowl and nominal orbital speeds (rpm) of low = 61, medium = 113, high = 205, Hobart Corp.
National cookie dough micromixer and special cookie dough bowl. National Manufacturing Co., a division of TMCO Inc.
Cookie sheets, gauge strips, and cutter. Wayne Machine Shop, Inc.
Refractory sheet. ZIRCAR Refractory Composites, Inc.


10-90.01, Baking Quality of Cake Flour

Electric mixer. Such as Hobart C-100, with 3-qt (2.8-liter) bowl or 5-qt (4.7-liter) bowl and nominal orbital speeds (rpm) of low = 61, medium = 109, high = 190, or Hobart N50 with 5-qt (4.7-liter) bowl and nominal orbital speeds (rpm) of low = 61, medium = 125, high = 259, Hobart Corp., or KitchenAid KSM 150PSOB, with 5-qt (4.7-liter) bowl and nominal orbital speeds (rpm) of stir speed = 61, speed no. 2 = 95, speed no. 6 = 180, KitchenAid


11-10.01, Bt Cry1Ab-Modified Corn in Corn Flour-ELISA Method

Reagents 1-8. Available as GMO Bt Maize Kit, Cat. No. SDI 711000, Strategic Diagnostics Inc.


11-20.01, StarLink Corn in Corn Flour and Corn Meal-ELISA Method

Reagents 1-8. Available as GMO Bt Maize Kit, Cat. No. SDI 7110030, Strategic Diagnostics Inc.


11-21.01, ELISA Method for StarLink Corn in Corn Flour and Corn Meal

Reagents 1-8 Available as QuantiPlate Kit for Cry9C, Cat. no AP 008, EnviroLogix, Inc.


11-30.01, Quantification of MON 810 Corn in Corn Flour by Real-Time Polymerase Chain Reaction

ABI PRISM 96-well optical reaction plate and MicroAmp optical caps, Cat. No. 403012 (or equivalent), Applied biosystems
GENESpin DNA extraction kit, Product Management Kits Cat. No. 5224400605, Eurofins GeneScan GmbH
Reagents, DNA polymerase (AmpliTaq Gold), deoxyribonucleotide triphosphates, TaqMan Buffer A, Uracil N-glycosylase (AmpErase), TaqMan PCR Core Reagents Kit, Cat. No.N8080228. Applied Biosystems
Calibration standards, 5% MON 810 DNA CRM (ERM code BF413c). ERM (ERM is the registered trademark for European Reference Materials Institute for Reference Materials and Measurements [IRMM]).


12-10.01, Residual Carbon Dioxide in Baking Powder

Chittick carbon dioxide apparatus. Mid Rivers Glassblowing, Inc., or available as Cat. No. 12-4425-01. Taylor Scientific


12-20.01, Total (Gasometric) Carbon Dioxide in Baking Powder

Chittick carbon dioxide apparatus. Mid Rivers Glassblowing, Inc., or available as Cat. No. 12-4425-01. Taylor Scientific


12-21.01, Total Carbon Dioxide in Prepared Mixes and Self-Rising Flours

Chittick carbon dioxide apparatus. Mid Rivers Glassblowing, Inc., or available as Cat. No. 12-4425-01. Taylor Scientific


14-10.01, Pekar Color Test (Slick Test)

Flour slick. No. 471, Seedburo Equipment Co.


14-22.01, Color of Pasta-Reflectance Colorimeter Method

Precision photoelectric reflectance colorimeter. Agtron model M-500A reflectance spectrophotometer, Agtron, Inc., Byk-Gardner

Hunter color difference meter, model D25, HunterLab, or Minolta color difference meter, Minolta Corp. USA, or Zeiss Ehepho relectance photometer, Carl Zeiss Vision, GmbH


14-30.01, Agtron Color Test for Flour

Agtron, models M-45, M-Series, M40, M-400-A, M-500-A, F-2-61, Agtron Inc.


14-50.01, Determination of Pigments

Laboratory mill. Falling Number KT-3100, Perten Instruments, Inc. or Cyclone, Udy Corp.


20-01.01, Egg Solids-Digitonin Cholesterol Method

Extraction tube,, Knorr type, containing medium-porosity fritted glass disk. Ace Glass, no. 7195-08, Ace Glass, Inc.


20-10.01, Egg Solids in Pasta Products

Filter. Allihn fritted glass, coarse porosity, with 10-cm stem (Ace Glass no. 7195-06), Ace Glass, Inc.


20-20.01, Determination of Isoflavones in Soy and Selected Foods Containing Soy by Extraction, Saponification, and Liquid Chromatography

Isoflavones standards. LC Laboratories, ChromaDex, or Sigma-Aldrich


22-02.01, Measurement of α-Amylase in Plant and Microbial Materials Using the Ceralpha Method

Reagents 1, 5, and 6. Available as Ceralpha α-Amylase Assay Kit, Cat. no. K-CERA, Megazyme International Ireland Ltd.


22-05.01, Measurement of α-Amylase in Cereal Grains and Flours- Amylazyme Method

Micropipets, 1.0-ml (Gilson Pipetman). P. Gilson
Positive displacement dispenser (Eppendorf multipette 4780 or Brinkmann Universal Pipette Dispenser). Brinkmann Instruments, Inc.
Vortex mixer (Maxi Mix II). Barnstead International, part of Thermo Fisher Scientific
Centrifugal mill. Fritsch GmbH
Incubation bath (Multistir incubation bath Mk III). Megazyme International Ireland Ltd.
Magnetic stirrer (KMO2 basic). IKA Works, Inc.
Amylazyme tablets. (Cat. No.: T-AMZ200) Megazyme International Ireland Ltd.
Trizma base solution 2% w/v. Sigma Chemical Co.


22-08.01, Measurement of α-Amylase Activity with the Rapid Visco Analyser

Rapid Visco Analyser, models RVA-Super 4, RVA-4, or RVA-TecMaster or RVA-Starchmaster2 and obsolete models. Perten Instruments, Inc.
Mill. Cyclotec model 1093 with 0.5-mm screen, sold by Foss North America Laboratory/factory mill, 6000 series with 0.8-mm screen, Perten Instruments, Inc., Mikro Sample Mill Pulverizer with 0.5-mm sieve, Hosokawa Micron Powder System; Laboratory Mill LM 3303/3310, LM 3100, LM 3600/3610, or LM 120, Perten Instruments, Inc.; or Udy Cyclone with 0.5-mm screen, Udy Corp


22-10.01, Measurement of α-Amylase Activity with the Amylograph

Visco/Amylo/Graph. C.W. Brabender Instruments, Inc.


22-12.01, Measurement of α-Amylase Activity in Flour Supplemented with Fungal α-Amylase-Modified Amylograph Method

Visco/Amylo/Graph, with 700 CMGM sensitivity cartridge. C.W. Brabender Instruments, Inc.
Pregelantinized wheat starch. American Ingredients Co.


22-62.01, Measurement of Proteolytic Activity-Spectrophotometric Method

Hemoglobin substrate powder, Cat. no. LS002402. Worthington Biochemical Corp.


22-80.01, Qualitative Test for Peroxidase in Oat Products

D-Araboascorbic acid (D-isoascorbic acid) (Cat. no AC22727-1000), 2,6-Dichloro-indophenol sodium salt (Cat. no. S286-5), Hydrogen Peroxide, 30% (Cat no. H325-100). Fisher Scientific, part of Thermo Fischer Scientific


22-85.01, Measurement of Polyphenol Oxidase in Wheat Kernels (L-DOPA Substrate)

Microcentrifuge tubes 2-ml polypropylene. Sigma T-3531, Sigma Chemical Co., www.sigmaaldrich.com; or Fisher 02-681332, Fisher Scientific
Tube shakers Fisher Cat. no 13-687-12 (Labquake shaker), Fisher Scientific
VWR Cat. no. 56264-306, VWR Scientific Products or Thermolyne Cat. no. 415-110, Barnstead International, part of Thermo Fisher Scientific
3-(N-Morpholino)propanesulfonic acid (MOPS), Sigma M-3183, Sigma Chemical Co.
L-Dihydroxyphenylalanine (L-DOPA), Sigma D-9628, Sigma Chemical Co.
Polyoxyethylenesorbitan monolaurate (Tween-20), Sigma P-7949, Sigma Chemical Co.


26-10.02, Experimental Milling: Introduction, Equipment, Sample Preparation, and Tempering

Bran finisher, experiemental. Bühler, Inc., or C. W. Brabender Instruments, Inc.,


26-21.02, Experimental Milling-Bühler Method for Hard Wheat

Laboratory pneumatic mill (Bühler model MLU-202), and laboratory impact finisher (Bühler model MLU-302). Bühler, Inc.,
Laboratory impact finisher (Brabender bran duster). C. W. Brabender Instruments, Inc.,


26-22.01, Experimental Milling-Batch Method for Hard Wheat

Roll stands. Kice Indusries, Inc
Laboratory impact finisher. Brabender bran duster, C. W. Brabender Instruments, Inc., or Bühler model MLU-302, Bühler, Inc.,


26-30.02, Experimental Milling-Bühler Method for Soft Wheat Short-Extraction Flour

Laboratory pneumatic mill. Bühler model MLU-202, Bühler, Inc.,


26-31.01, Experimental Milling-Bühler Method for Soft Wheat Straight-Grade Flour

Laboratory mill. Bühler model MLU-202, Bühler, Inc.,


26-32.01, Experimental Milling-Batch Method for Soft Wheat

Roll stands. Kice Indusries, Inc


26-41.01, Experimental Milling-Bühler Method for Durum Wheat

Laboratory pneumatic mill. Bühler model MLU-202, Bühler, Inc.,


26-42.01, Experimental Milling-Batch Method for Durum Wheat

Roll stands. Kice Indusries, Inc


26-50.01, Brabender Quadrumat Jr. (Quadruplex) Method

Brabender Quadrumat Jr. laboratory mill and semolina version of the Quadrumat Jr. II. C. W. Brabender Instruments, Inc., or Great Western Manufacturing Co., Inc., (this company will reclothe Quadrumat Jr. reels)


26-70.01,Chopin CD1 Laboratory Mill Method

Laboratory mill, Chopin CD1, Chopin Technologies
Mechanical cleaner, Carter Day dockage tester
Tempering mixer system, Chopin Technologies


28-01.01, Apparatus or Materials for Extraneous Matter Methods

Rod and disk assembly for trap flask.
Autoclave, Filter paper (lined S&S no. 8, Cat. no. 2844-067), Büchner funnel (Cat. no. 30310-038), petri dish (10-mm, Cat. no. 25384-208), trap flask, probes, magnetic stir bar, shaker, Jones sampler, cutting mill, muffle furnace, beakers, sieves. VWR Scientific Products


28-02.01, Reagents for Extraneous Matter Methods

Mineral oil 125/135 Saybolt universal viscosity, specific gravity 0.840-0.846, Cat. no. 1443952. U.S. Pharmacopeia Convention, Inc., Reference Standards
Xanthydrol (9-Hydroxy Xanthene 99% syn. Xanthydrol Cat. no. AC 271520050) Fisher Scientific


28-06.01, Cinder and Sand Particles in Farina-Counting Method

Büchner funnel (Cat. no. 30310-038), petri dish (10-mm, Cat. no. 25384-208), Filter paper (lined S&S no. 8, Cat. no. 2844-067), beaker. VWR Scientific Products


28-07.01, Cinder and Sand Particles in Farina-Gravimetric Method

Separatory funnel (Pyrex centrifuge, 125-ml), crucible, muffle furnace, Whatman no. 40 ashless Filter paper, desiccator, beaker (Cat. no. 284-75-004) VWR Scientific Products


28-19.01, External Filth and Internal Insect Infestation in Whole Corn

Blender (Osterizer, Cat. no. 58977-033), Kilborn funnel, wrist-action shaker (Cat. no. 57040-049), hot plate/stirrer, Büchner funnel (Cat. no. 30310-038), beaker (1.5 liter, Cat. no. 13910-303), petri dish (10-mm, Cat. no. 25384-208), squeeze bottle (Cat. no. 16651-610), Filter paper (S&S no. 8, Cat. no. 2844-067), probe. VWR Scientific Products


28-20.02, Microscopic Examination of External Contamination in Whole Grains

Trap Flask (2-liter), hot plate, probe, petri dish, beaker, Filter paper (S&S no. 8, Cat. no. 2844-067). VWR Scientific Products


28-21.02, X-Ray Examination for Internal Insect Infestation

Jones Sampler (Cat. no. 56720-081). VWR Scientific Products


28-22.02, Cracking-Flotation Test for Internal Insects in Whole Grains

Sampler, trap flask (2-liter), probe petri dish, squeeze bottle. VWR Scientific Products


28-31.02, Pancreatin Sieving Method, for Insect and Rodent Filth in Materials Hard to Hydrate

Beaker (2-liter, Cat. no. 13912-648), sieve (no. 140, 8-in., Cat. no. 57320-840), wash bottle (Cat. no. 16651-609), probe, petri dish (10-mm, Cat. no. 25384-208), trap flask, Filter Paper (S&S no. 8, Cat. no. 2844-067), Büchner funnel (Cat. no. 30310-038), pH meter, litmus paper. VWR Scientific Products


28-32.02, Sieving Method, for Materials Hard to Hydrate

Beaker (1500-ml, Cat. no. 13910-303), sieve (no. 14, 8-in., Cat. no. 57320-544), wash bottle (Cat. no. 16651-609), probe, petri dish (10-mm, Cat. no. 25384-208), trap flask, Filter Paper (S&S no. 8, Cat. no. 2844-067), Büchner funnel (Cat. no. 30310-038), pH meter, litmus paper. VWR Scientific Products


28-33.02, Pancreatin Nonsieving Method for Insect and Rodent Filth in Materials Hard to Hydrate

Beaker (1500-ml, Cat. no. 13910-303), sieve (no. 14, 8-in., Cat. no. 57320-544), wash bottle (Cat. no. 16651-609), probe, petri dish (10-mm, Cat. no. 25384-208), trap flask, Filter Paper (S&S no. 8, Cat. no. 2844-067), Büchner funnel (Cat. no. 30310-038), pH meter, litmus paper. VWR Scientific Products
Glass stir rod Fisher Scientific


28-40.01, Acid Hydrolysis Method for Insect Fragments and Rodent Hairs- Wheat-Soy Blend

Sieve (no. 230, 8-in., Cat. no. 57320-908), separatory funnel, beaker (1-liter, Cat. no. 13912-604), magnetic stirrer and stir bar, retriever, probes, wash bottle (Cat. no. 16651-609), wide-mouth glass funnel (Cat. no. 30242-100), probe, Büchner funnel (Cat. no. 30310-038), petri dish (10-mm, Cat. no. 25384-208), Filter paper (S&S no. 8, Cat. no. 2844-067). VWR Scientific Products
Glass stir rod Fisher Scientific


28-41.03, Acid Hydrolysis Method for Extracting Insect Fragments and Rodent Hairs-Light Filth in White Flour

Beaker (Cat. no. 13912-604), Petri Dish (Cat. no. 25384-208), Filter Paper (Cat. no. 2844-067), Stir Bar (Cat. no. 58948-988), retriever (Cat. no. 58949-280), squeeze bottle (Cat. no. 16651-610). VWR Scientific Products


28-44.01, Iodine Method, for Insect Eggs in Flour

Sieve (no. 100, 8-in., Cat. no. 57320-806), hot plate, beaker (250-ml, Cat. no. 13910-201), Büchner funnel (Cat. no. 30310-038), Filter paper (S&S no. 8, Cat. no. 2844-067), squeeze bottle, petri dish (10-mm, Cat. no. 25384-208). VWR Scientific Products


28-50.01, Decantation Method, for Rodent Excreta

Beaker (250-ml, Cat. no. 13910-201), wash bottle (Cat. no. 16651-609), probes, petri dish (10-mm, Cat. no. 25384-208), Filter paper (S&S no. 8, Cat. no. 2844-067), Büchner funnel (Cat. no. 30310-038). VWR Scientific Products


28-51.02, Flotation Method, for Insect and Rodent Filth

Trap Flask (1-liter), Wash Bottle (Cat. no. 16651-609), probes, petri dish (10-mm, Cat. no. 25384-208), Filter paper (S&S no. 8, Cat. no. 2844-067), Büchner funnel (Cat. no. 30310-038). VWR Scientific Products


28-60.02, Tween-Versene Method, for Insect Fragments and Rodent Hairs in Rye Flour

Beaker (2-liter, Cat. no. 13912-648), wash bottle (Cat. no. 16651-609), probes, petri dish (10-mm, Cat. no. 25384-208), Filter paper (S&S no. 8, Cat. no. 2844-067), Büchner funnel (Cat. no. 30310-038). VWR Scientific Products


28-70.01, Defatting-Digestion Method, for Insect Fragments and Rodent Hairs

Beaker (600-ml, Cat. no. 13910-245), hot plate VWR Scientific Products


28-75.02, Sieving Method, for Light Filth in Starch

Beaker (2-liter, Cat. no. 13912-648), sieve (no. 230, Cat. no. 57320-908), wash bottle (Cat. no. 16651-609), probes, petri dish (10-mm, Cat. no. 25384-208), Büchner funnel (Cat. no. 30310-038). VWR Scientific Products


28-80.01, Flotation Method, for Insect and Rodent Filth in Popped Popcorn

Trap Flask VWR Scientific Products


28-87.01, Urease-Bromthymol Blue Test Paper, for Urea

Filter Paper (S&S no. 5, Cat. no. 598 or 599), watch glass. VWR Scientific Products


30-20.01, Crude Fat in Grain and Stock Feeds

Whatman extraction Thimbles. Cat. no. 27730-060, VWR Scientific Products


30-25.01, Crude Fat in Wheat, Corn, and Soy Flour, Feeds, and Mixed Feeds

Whatman extraction Thimbles. Cat. no. 27730-060, VWR Scientific Products


32-05.01, Total Dietary Fiber

Enzymes for TDF assays. Celite (Cat. no. C211), Fisher Scientific or a-Amylase, heat stable (cat.no. E-BLAAM), protease (Cat. no. E-BSPRT), amyloglucosidase (Cat. no. E-AMGDF); Celite (Cat. no. G-CEL100, G-CEL500); or TDF assay kit (Cat. no. K-TDFR), Megazyme International Ireland Ltd. or α-amylase, heat stable (Cat. no. A 3306), protease (cat. no. P 3910), amyloglucosidase (Cat. no. A 9913); Celite (Cat. no. C 8656); or TDF assay kit, (Cat no. TDF-100A), Sigma Chemical Co.


32-06.01, Total Dietary Fiber-Rapid Gravimetric Method

Extraction system. Fibertec 1020 and 2010, with incubation flask, Foss North America
Incubation flasks. Tecator P2 crucible.Foss North America
Enzymes for TDF assays. Protease (Cat. no. E-BSPRT), amyloglucosidase (Cat no. E-AMGDF), Celite (Cat. no. G-CEL100, G-CEL500). Megazyme International Ireland Ltd.
Enzymes for TDF assays. α-amylase, type VI-B from hog pancreas, heat stable (Cat. no. A 3176), protease (Cat. no. P 3910), amyloglucosidase (Cat. no. A 9913), Celite (Cat no. C 8656). Sigma Chemical Co.
Celite, Filtering aid (Fisher, Cat. no. C-211500). Fisher Scientific


32-07.01, Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products

Enzymes for TDF assay: α-amylase, heat-stable (Cat. no. E-BLAAM), protease (Cat. no. E-BSPRT), amyloglucosidase (Cat. no. E-AMGDF), Celite (Cat. no. G-CEL100, G-CEL500); or TDF assay kit (Cat. no. K-TDFR). Megazyme International Ireland Ltd.,
Enzymes for TDF assay: α-amylase, heat stable A 5426 (Cat. no. A 3306), protease (Cat. no. P 3910), amyloglucosidase (Cat. no. A 9913), Celite, (Cat. no. C 8656); or TDF assay kit (Cat. no. TDF-100A); 2(N-morpholino)-ethanesulfonic acid (Cat. no. M 8250); and tris(hydroxymethyl)amino-methane (Cat. no. T 1503). Sigma Chemical Co.


32-10.01, Crude Fiber in Flours, Feeds, and Feedstuffs

Laboratory mill with 1-mm screen. Micromill, with screen 1/25–1/16 in. No. 18 or 12, Hosokawa Micron Powder Systems, or Weber mill with screen, 0.033–0.040 in. No. 18 or 20, Weber S-500, Weber Brothers
Digestion apparatus (models 30001 and 30002). Labconco Corp.
Filter W. S. Tyler Assoc.
Modified California State Büchner funnel. Cat. no. 4280, Nalge Nunc International Corp.
Ceramic fiber sheets. Cat. no. WLS 43702-20A, VWR Scientific Products
Antifoam. Dow Corning Corp.


32-20.01, Insoluble Dietary Fiber

Enzyme for IDF assays: α-amylase. Cat. no. E-BLAAM, Megazyme International Ireland Ltd., or type VI-A from hog pancreas (Cat. no. A-3176), Sigma Chemical Co.


32-21.01, Insoluble and Soluble Dietary Fiber in Oat Products-Enzymatic-Gravimetric Method

Celite, acid-washed, incinerated, Fisher Scientific,
Enzymes for IDF and SDF assays: α-amylase, heat stable (cat. no. E-BLAAM), proteas (Cat. no. E-BSPRT), amyloglucosidase (Cat no. E-AMGDF), Celite (Cat. no. G-CEL100, G-CEL500); or TDF assay kit (Cat. no. K-TDFR). Megazyme International Ireland Ltd.,
Enzymes for IDF and SDF assays: α-amylase, heat stable (Cat. no. A 3306), protease (Cat. no. P 3910), amyloglucosidase (Cat. no. A 9913), Celite (Cat no. C 8656); or TDF assay kit (Cat. no. TDF-100A). Sigma Chemical Co.


32-22.01, ß-Glucan in Oat Fractions and Unsweetened Oat Cereals

Grinding mill. Retsch ZM200 (Cat. no. 208230003) with 12 tooth stainless steel (Cat. no. 026080041) with .5mm stainless steel screen (Cat. no. 036470235). Retsch Inc.,
Centrifuge, (Sorvall RC-6) with rotor to hold 50-ml polypropylene tubes. Thermo Scientific, part of Thermo Fischer Scientific
Benchtop centrifuge (optional), Thermo Scientific IEC Multi Centrifuge (Cat. no. 05-375-74) with 50-ml rotor (Cat. no. 05-375-79). Fisher Scientific, part of Thermo Fischer Scientific
Micro-centrifuge (optional), Eppendorf 5415D (Cat. no. 05-40-100). Fisher Scientific, part of Thermo Fischer Scientific
Polypropylene centrifuge tubes with caps, (Cat. no. 05-563-10G). Fisher Scientific, part of Thermo Fischer Scientific
Spectrophotometer, equipped with sipper cell Hitachi U-3210,
Hitachi High Technologies America, Inc.
Incubation bath. Incubation bath MK III (Cat. no. G-IBMKIII). Megazyme International Ireland Inc.
D-Glucose, anhydrous. (Cat. no. 16325), corn starch (Cat. no. S2388), wheat starch (Cat. no. S1514), and 4-aminophenazone (4- aminoantipyrine) (Cat. no. A4382). Sigma Chemical Co.
Glucose oxidase/peroxidase reagent, available in Glucose Assay Kit (Cat. no. K-GLUC), β-Glucan Assay Kit (Cat. no. K-BGLU). Megazyme International Ireland Ltd.
Glucose oxidase, (250U/mg) (Cat. no. 000000) and Peroxidase (250U/mg) (Cat. no. 1010809001). Roche Diagnostics


32-23.01, β-Glucan Content of Barley and Oats-Rapid Enzymatic Procedure

Grinding mill. Fritsch centrifugal, Fritsch GmbH. Dealer: Gilson Co., Inc.
Sample mill (Tecator Cyclotec 1093). Foss North America
Mixed linkage β-glucan assay kit (Cat. no. K-BGLU); lichenase (EC 3.2.1.73) 5000U (Cat. no. E-LICHN); β-glucosidase (EC 3.2.1.21) 200U (Cat. no. E-BGLUC); glucose assay kit (Cat. no. K-GLUC); D-glucose stan-dards (supplied with both assay kits); and Multistir incubation bath MK111 (Cat. no. IBM K111). Megazyme International Ireland Ltd.
D-Glucose, crystalline (Sigma, Cat. no. G 5250) and corn starch (Cat. no. S 1514). Sigma Chemical Co.


32-24.01, β-Glucan Viscosity in Cereal Products with the Rapid Visco Analyzer

Bacterial β-amylase (Bacillus licheniformis), E-BLAAM100, Megazyme International Ireland Ltd.
Bacterial protease (B. licheniformis), E-BSPRT100, Megazyme International Ireland Ltd.
Lichenase, E-LICHN, Megazyme International Ireland Ltd.
Rapid Visco Analyzer, model RVA-Super3, RVA-4, RVA-Super4, or RVA 4500, Newport Scientific
Universal mill, model M2, IKA-Werke


32-25.01, Total Dietary Fiber-Determined as Neutral Sugar Residues, Uronic Acid Residues, and Klason Lignin (Uppsala Method)

Mill, cyclone. Cyclotec 1093, Foss North America
Capillary column. Cat. no. DB-225, Agilent Technologies
amyloglucosidase, E.C. 3.2.1.3. Cat. no. 1202 367, Roche Diagnostics, or No. E-AMGDF, Megazyme International Ireland Ltd.
Termamyl, E.C343. 3.2.1.1 (heat-stable α-amylase) solution. No. E-BLAAM, Megazyme International Ireland Ltd. or Novozymes
Commercial polysaccharides: arabinoxylan from wheat flour and β-glucan from oat (Cat. no. P-WAXYM or P-BGOM). Megazyme International Ireland Ltd.
Commercial polysaccharides: citrus pectin (Sigma P 9135) and wheat starch (S 1514). Sigma Chemical Co.


32-28.02, Polydextrose in Foods by Ion Chromatography

High-speed centrifuge and rotors. J2-21 centrifuge with the JA-17, JA-18.1, and JA-25.15 rotors, Beckman Instruments, Inc.
Centrifuge tubes, 50-ml (holding less than 38 ml), capable of withstanding 38,000 × g (Cat. no. 3103-0050). Nalge Nunc International Corp.
Centrifugal ultrafiltration devices, with 100,000 NMWL, polyethersulfone membrane, 2-ml capacity (Cat. no. 4104). Ultrafree-CL PBHK BIOMAX-100, Millipore
Filters, PTFE, syringe, 0.2-µm pore size, 13-mm diameter. Fisher Scientific
Liquid chromatography and detectors, high-pressure anion-exchange chromatography with electrochemical detection. DX 500 basic gradient carbohydrate system. Dionex Corp.
LC column, 250 × 4 mm. CarboPak PA1, Dionex Corp.
Guard column. CarboPak PA1 Guard, 23 × 3 mm, Dionex Corp.
Enzymes: fructanase, amyloglucosidase, and isoamylase. E-FRMXLQ (fructanase), E-AMGDF (amyloglucosidase), and E-ISAMY (isoamylase), Megazyme International Ireland Ltd.


32-31.01, Fructans in Foods and Food Products-Ion Exchange Chromatographic Method

High-performance anion-exchange chromatograph. Dionex 4500i LC gradient pump with equipment including Carbopac PA1 anion-exchange resin and Carbopac PA guard column, Dionex Corp.
Enzymes for assays:amyloglucosidase. Cat. no. E-AMGDF, Megazyme International Ireland Ltd.
Enzymes for assays: Fructozyme. Novozymes
Reagents and enzymes for assays: maltitol (Cat. no. M-8892) and amyloglucosidase (Cat. no. A 7420). Sigma Chemical Co.


32-32.01, Measurement of Total Fructan in Foods by an Enzymatic/Spectro-photometric Method

Fructan assay kit (Cat. no. K-FRUC, including reagents 7–11). Megazyme International Ireland Ltd.
p-Hydroxybenzoic acid hydrazide (Cat. no. H-9882) and sodium borohydride (Cat. no. S-9125). Sigma Chemical Co.


32-33.01, Determination of trans-Galactooligosaccharides in Selected Food Products by Ion-Exchange Chromatographic Method

Automated sampler. Dionex Corp.
Data integrator. Shimadzu America/Shimadzu Scientific Instruments, Inc.
Galactosidase from Aspergillus oryzae. Lactase F, Amano Enzyme U.S.A. Co., Ltd.


32-40.01, Resistant Starch in Starch Samples and Plant Materials

Shaking water bath. Model OLS 200, Grant Instruments Cambridge Ltd.
Culture tubes. Corning , screw cap, 16 × 125 mm, cat. nos. TKV-173-030B (tubes); TKV-178-020V (caps); Fisher Scientific
Resistant Starch Assay Kit. Megazyme International Ireland Ltd.


32-41.01, Total Dietary Fiber in Foods Containing Resistant Maltodextrin-Enzymatic-Gravimetric Method and Liquid Chromatography Determination

Guard column, 6.0 mm i.d. x 4 cm. TSK PWXL, Tosoh Corp.
LC columns, two, connected in series, 7.8 mm i.d. x 30 cm. TSK-GEL G2500PWXL, Tosoh Corp.
Rotary evaporator. R-3000VW 'Student,' Bùchi Labortechnik AG
Heat-stable alpha-amylase solution, activity: 12 U/mg protein; should not contain glycerol. Termamyl, no. 120L, Novo Nordisk A/S
LC retention time standard, standard source of the distribution of oligosac-charides (DP 3) in the LMW-RMD fraction of RMD. Fibersol-2, Matsutani Chemical Industry Co., Ltd.
Corn syrup solids, DE 25. Matsutani Chemical Industry Co., Ltd.
Protease, activity: 7-15 U/mg protein; should not contain glycerol. P-3910 or P-5380, Sigma-Aldrich Chemical Co.
Amyloglucosidase, activity: 400 U/mg protein; should not contain glycerol. A-9913, Sigma-Aldrich Chemical Co.
Celite. C-8656, Sigma-Aldrich Chemical Co., or C-211, acid washed, Fisher Scientific
Mixed-bed ion exchange resins for each test portion. 1) Amberlite IRA-67 (OH-type), and 2) Amberlite 200 CT(HG)H (H-type), Organo Corp.
Alternative mixed-bed ion exchange resins (from Note 2). Amberlite 200 (Na-type). Sigma-Aldrich Chemical Co.,, or Amberlite 200CT, Organo Corp


32-45.01, Total Dietary Fiber (Codex Alimentarius Definition)

Fritted crucibles. . Corning no. 36060-60C, or equivalent. Corning Incorporated
Guard column. Part no. WAT015209, Waters Corp.; TSK Gel PWxl, www.sigmaaldrich.comSigma Chemical Co.
HPLC columns. Waters Sugar-Pak, part no. WAT085188, Waters Corp.
Shaking incubation bath. Cat. no. OLS 200, Grant Instruments
Size-exclusion LC columns. TSK-Gel, G2500PWXL, part no. 808020, Sigma Chemical Co.
Acetic acid solution. Fluka 45731. Sigma Chemical Co.
Barley beta-Glucan. P-BGBM, Megazyme International Ireland Ltd.
Celite. G-CEL100 or G-CEL500, Megazyme International Ireland Ltd.
D-Glucose LC standards. Cat. no. G8270 or D9434, www.sigmaaldrich.comSigma Chemical Co.
Integrated Total Dietary Fibre Assay Kit (K-INTDF). Megazyme International Ireland Ltd.
Ion exchange resins. Amberlite FPA53 and Ambersep 200, Dow; G-AMBOH and G-AMBH, Megazyme International Ireland Ltd.
LC retention time standard. Matsutani Chemical Industry Co.
Maltodextrin. TK-16, Matsutani Chemical Industry Co.; Cat. no. 419699, maltodextrin with 16.5–19.5 dextrose equivalents, Sigma Chemical Co.; Megazyme International Ireland Ltd.
Micro-90 cleaning solution. International Products Corp.
Tris buffer salt. Cat. no. B-TRIS500, Megazyme International Ireland Ltd.; Cat. no.T-1503, Sigma Chemical Co.
Trizma base. Cat. no. T-1503. <Sigma Chemical Co.


38-12.02, Wet Gluten, Dry Gluten, Water-Binding Capacity, and Gluten Index

Glutomatic system; laboratory mill (LM 3100 or LM 120, with 0.8-mm screen); and centrifuge sieves and centrifuges. Perkin Elmer


38-20.01, Farinograph Test for Vital Wheat Gluten

Farinograph. C. W. Brabender Instruments, Inc., or Brabender OHG


38-50.01, Gluten in Corn Flour and Corn-Based Products by Sandwich ELISA

RIDASCREEN Gliadin, art. no. R7001, R-Biopharm
RIDASOFT Win, art. no. Z9999, R-Biopharm
Cocktail (patented), art. no. R7006, R-Biopharm


38-55.01, Gluten in Fermented Cereal-Based Products by Competitive ELISA

Fish gelatin, liquid, art. no. 22156, Serva; or art. no. G-7765, Sigma
RIDASCREEN Gliadin competitive, art. no. R7021, R-Biopharm
RIDASOFT Win, art. no. Z9999, R-Biopharm


38-60.01, Gluten in Corn Flour and Corn-Based Products by Qualitative R5 Analysis

Cocktail, (patented, WO/2002/092633), R7006, R-Biopharm
RIDA®QUICK Gliadin, , R5 antibody test kit, R-Biopharm


39-10.01, Near-Infrared Reflectance Method for Protein Determination in Small Grains

Near-infrared reflectance instrumentation. Bran+Luebbe, Inc.; Dickey-john Corp.; Foss NIRSystems or Foss North America; Leco Corporation; Verum Analytics; ; Shimadzu American/Shimadzu Scientific; or Zeltex, Inc.


39-20.01, Near-Infrared Reflectance Method for Protein and Oil Determination in Soybeans

Mill, with 3/4-horsepower motor, belt drive, screen with 2-mm round hole, and automatic 13-rpm feeder. Udy Cyclone, Udy Corp.
Sieves. Seedburo Equipment Co.


39-21.01, Near-Infrared Reflectance Method for Whole-Grain Analysis in Soybeans

Near-infrared instrument. Infratec model 1229, Foss North America
Sieves. Seedburo Equipment Co.


39-25.01, Near-Infrared Reflectance Method for Protein Content in Whole-Grain Wheat

Laboratory mill. Cyclone, Udy Corp.,
Near-infrared spectrophotometer. Foss North America; Perkin Elmer
Natural products cell. Infrasoft International, LLC,


39-70.02, Near-Infrared Reflectance Method for Hardness Determination in Wheat

Lab mill. Foss North America; Perkin Elmer; or Udy Corp.
Standard wheat hardness samples. National Institute of Standards and Technology (NIST)


40-10.01, Aluminum-Qualitative Method

Aurintricarboxylic acid. Cat. no. A1895-SG, Sigma Chemical Co.


40-70.01, Elements by Atomic Absorption Spectrophotometry

Lanthanum (III) oxide, low calcium. Cat. no. 11266, Alfa æSAR
Standard reference material. National Institute of Standards and Technology (NIST)


40-71.01, Sodium and Potassium by Atomic Absorption Spectrophotometry

Standard reference material. National Institute of Standards and Technology (NIST)


40-75.01, Determination of Minerals by Inductively Coupled Plasma Spectroscopy

Cesium chloride. Cat. no. C24-25, Fisher Scientific
Triton X-100 Lab Chem. Cat no. NC9903183, Lab Chem., distributed by Fisher Scientific
Standard reference material.National Institute of Standards and Technology (NIST)


42-11.01, Aerobic Plate Count

Stomacher. Lab Blender 400, Seward Medical Ltd.; supplier (Cat. no. 10-0541-000): Tekmar-Dohrmann
Colony counter. Dark-field, Leica/Quebec, Cat. nos. 3325 for standard, 3327 for electronic, Leica Microsystems Inc., Educational and Analytical Division


42-15.02, Coliform-E. coli

Stomacher. Lab Blender 400, Seward Medical Ltd.; supplier (Cat. no. 10-0541-000): Tekmar-Dohrmann


42-17.02, Clostridium perfringens Enumeration

Stomacher. Lab Blender 400, Seward Medical Ltd. ; supplier (Cat. no. 10-0541-000): Tekmar-Dohrmann
Plain membrane disposable Filter unit, 0.45 µm. Cat. no. 28199-064, Nalge Nunc International Corp.


42-20.01, Rope Spore Count

Stomacher. Lab Blender 400, Seward Medical Ltd. ; supplier (Cat. no. 10-0541-000): Tekmar-Dohrmann, Tekmar-Dohrmann
Colony counter. Dark-field, Leica/Quebec, Cat. nos. 3325 for standard, 3327 for electronic, Leica Microsystems Inc., Educational and Analytical Division


42-25.03, Salmonella Bacteria

Filter, 0.45 µm. 250 ml Filter system, 0.45u CA (cellulose acetate), (Cat. no. 430 768, Corning Incorporated; or plain membrane disposable Filter unit, Nalge Nunc International Corp.
Stomacher. Lab Blender 400, Seward Medical Ltd.; supplier (Cat. no. 10-0541-000): Tekmar-Dohrmann


42-30.04, Enumeration of Staphylococcus aureus

Stomacher. Lab Blender 400, Seward Medical Ltd.; supplier (Cat. no. 10-0541-000): Tekmar-Dohrmann
Egg yolk tellurite enrichment. Cat. no. 277910, Becton Dickinson Diagnostic Systems


42-35.01, Enterococci

Stomacher. Lab Blender 400, Seward Medical Ltd.; supplier (Cat. no. 10-0541-000): Tekmar-Dohrmann
Colony counter. Dark-field, Leica/Quebec, Cat. nos. 3325 for standard, 3327 for electronic, Leica Microsystems Inc., Educational and Analytical Division


42-40.01, Thermophilic Spore Counts (Total Aerobic, Flat-Sour, H2S, Non-H2S Anaerobic)

Stomacher. Lab Blender 400, Seward Medical Ltd.www.seward.co.uk; supplier (Cat. no. 10-0541-000): Tekmar-Dohrmann
Colony counter. Dark-field, Leica/Quebec, Cat. nos. 3325 for standard, 3327 for electronic, Leica Microsystems Inc., Educational and Analytical Division,
Alaska seed peas. Cat. no. 423, Sygenta Global, Seed Division


42-45.01, Thermophilic and Psychrotrophic Bacteria

Stomacher. Lab Blender 400, Seward Medical Ltd.; supplier (Cat. no. 10-0541-000): Tekmar-Dohrmann
Colony counter. Dark-field, Leica/Quebec, Cat. nos. 3325 for standard, 3327 for electronic, Leica Microsystems Inc., Educational and Analytical Division


42-50.02, Mold and Yeast Counts

Stomacher. Lab Blender 400, Seward Medical Ltd.www.seward.co.uk; supplier (Cat. no. 10-0541-000): Tekmar-Dohrmann
Colony counter. Dark-field, Leica/Quebec, Cat. nos. 3325 for standard, 3327 for electronic, Leica Microsystems Inc., Educational and Analytical Division
Filter, 0.45 µm. 250 ml Filter system, 0.45u CA (cellulose acetate), (Cat. no. 430 768, Corning Incorporated; or plain membrane disposable Filter unit, ThermoFisher Scientific Nalgene Labware
Chlortetracycline HCL (Cat. no. C-4881) or chloramphenicol (Cat. no. C1919). Sigma Chemical Co.


42-70.01, Smut-Microscopic Method

Hemacytometer. Cat. no. SI7036, Fisher Scientific


42-71.01, Smut-Sedimentation Method

Tween-20 (Cat. no. BP337) and Roccal (see Web page). Fisher Scientific


44-01.01, Moisture-Electrical Methods

Moisture tester. Steinlite Corp.


44-11.01, Moisture-Dielectric Meter Method

Moisture meter. Model 919, Dino's Labtronics


44-17.01, Moisture-Air-Oven Method (Pulses)

Laboratory mill. Thomas Wiley model ED5, Thomas Wiley Scientific; or Laboratory Mill LM 3303/3310, Perkin Elmer


45-05.01, Aflatoxin-Thin-Layer Chromatography Method

Device for preparing subsample. Dickens-Satterwhite mill, Georgia Federal State Inspection Service
Silica gel. Cat. no. EM 7734-3, for minicolumn, 0.05-0.2 mm, VWR Scientific Products
Aflatoxin standards. Various sources, including: BCR423RM, Sigma Chemical Co.
Divider, riffle-type. Seedburo model 106, Seedburo Equipment Co.


45-14.01, Aflatoxin-Rapid Screening Method

Longwave, 365-nm, ultraviolet light source. Chromato-Vue cabinet equipped with two 15-W lamps (model number C 71, Cat. no. 95002201) and Blak-ray lamp (model B-100-AP), UVP Inc.
Florisil. Cat. no. F-101, Fisher Scientific
Silica gel 60 (Cat. no. EM 7734-3) and Silica gel for minicolumn, 0.05-0.2mm. VWR Scientific Products
Neutral alumina, 80-100 mesh. Brockman activity, Cat. no. 0540-01, Biocompare
Device for preparing subsample. Georgia Federal State Inspection Service
Divider, riffle-type. Seedburo model 106, Seedburo Equipment Co.


45-15.01, Aflatoxin-Presumptive Test

UV source, 365-nm. Cole-Parmer Instrument Co.; or high-intensity Blak-ray lamp MB-100; black light cabinet equipped with two 15-W UV bulbs, no. 229; grain viewer, no. 228, <Seedburo Equipment Co.
Color reference standard. Tinapol BHS and Tinapol CBS-X, BASF Chemical Corp.


45-16.01, Aflatoxin-Liquid Chromatography Method

Liquid chromatograph (Varian model 9000 with fluorescence detector model 9070), 7-54 and 7-60 excitation Filters (360 nm); 3-73 and 4-76 glass emission Filters (440 nm); integrator, model 4400. Varian Inc. Scientific Instruments
LC Column, 15cm × 4.6mm inside diameter. Supelcosil LC-18 no. 58230-U, 5 µm, Sigma-Aldrich
Anhydrous ethyl ether. Mallinckrodt no. 0848, Biocompare
Silica gel. Cat. no. EM 7734-3, VWR Scientific Products


45-20.01, Zearalenone-Thin-Layer Chromatography Method

Device for preparing subsample. Georgia Federal State Inspection Service
Chromato-Vue cabinet, equipped with two 15-W lamps. Model C71, Cat. no. 95002201, UVP Inc.
Wrist action shaker (Burrell no. 14-260) and 4-dram, foil-lined screw-cap vials (Kimble Products no. 60910-L-4). Fisher Scientific
Fluorospectrometer. Hitachi model F-4500, Hitachi Instruments
Silica gel for TLC. Mallinckrodt Cat. no. 3407-01, Biocompare
Zearalenone. Sigma Z 2125, Sigma Chemical Co.
Riffle-type divider. Seedburo model 106, Seedburo Equipment Co.


45-21.01, Zearalenone and α-Zearalenol in Corn-HPLC Method

Planetary mixer. Hobart Corp.
Wrist action shaker. Burrell no. 14-260, Fisher Scientific
LC pump (Waters model 515 part number WAT207000) and injector (Rheodyne 7725 I). Waters Associates
Filter paper, fluted 24-cm S&S 588 rapid flow Filter paper. VWR catalog no. 28420-080, VWR Scientific Products
Sample Filters, e.g., Filter assembly and glass fiber Filters (Cat. no. 29440-024 and 28150-779, Pall Life Sciences, Inc.). VWR Scientific Products
Fluorescence detector. Model LC240, Perkin Elmer Corp.
Zearalenone standard, 1mg/1ml solution. Sigma Z 4950, Sigma Chemical Co.


45-25.01, Identification of Aflatoxin on TLC Plates

Desiccating storage cabinet. Fisher 8-545-5, Fisher Scientific
Hot air blower. Master Appliance Corp.
Silica gel for TLC. GHR silica gel, Macherey-Nagel GmbH & Co.; or Silic analytical reagent 4G or 7G, Biocompare
Aflatoxin standards. Various sources, including: Laboratory for Residue Analysis, National Institute of Public Health and the Environment; Romer Labs, Inc.; Sigma Chemical Co.


45-41.01, Deoxynivalenol in Wheat-TLC Method

Viewing cabinet. Chromato-Vue C-65, UVP Inc.
TLC plates (Cat. no. S75114) and Diatomaceous earth, Celite 545 JT3371-1. Fisher Scientific; or VWR Scientific Products
Activated charcoal. Darco, Cat. no. MCX06451, Fisher Scientific; or Biocompare


45-51.01, Fumonisins B1, B2, and B3 in Corn-Liquid Chromatographic Method

Solid-phase extraction cartridges. Varian Bond Elut, Varian Inc. Scientific Instruments
Fumonisin standards. The South African Medical Research Council (SAMRC)


46-09.01, Crude Protein-Automated Colorimetric Method

Block digestor (model BD-20), digestion tubes, auto-sampler, peristaltic pumping device, ammonia analytical cartridge, colorimeter, recorder, pump tubes, fittings, coil, and wetting solution. Bran+Luebbe, Inc.
Block digestor, capable of digesting 20 samples and maintaining 410°. Model DS-20 part no. TC2020-001, Foss North America


46-14.03, Crude Protein-Udy Dye Method

Protein analyzer for wheat, grains, etc. (SSP [React-R-Mill] or MSP [React-R-Shaker]), sample mill (Cyclone, Cat. no. 3010-030), standard Acid Orange 12 dye reagent, purified dye, and software. Udy Corp.


46-15.01, Crude Protein-5-Minute Biuret Method for Wheat and Other Grains

Sample mill. Cyclone, Udy Corp.


46-16.01, Crude protein-Improved Kjeldahl Method, Copper Titanium Dioxide Catalyst Modification

Antibumping agent, alundum, 8–14 mesh. Cat. no. 1590-D18, Thomas Scientific Co.


46-23.01, Nitrogen Solubility Inde

Glass wool. Pyrex brand Fiberglass, Corning no. 3950, Cat. no. 32848-003, VWR Scientific Products
Silicone antifoam. Dow Corning Corp.


48-62.01, Ammonium Persulfate-Quantitative Method

Fluorometer. Any instruments on market are suitable. For instance, Turner model 450, Cat. no. 07-118-124, Fisher Scientific
Filter paper. Reeve Angel no. 230, Cat. no. 09-803A, Fisher Scientific


54-10.01, Extensigraph Method, General

Farinograph and extensigraph. C. W. Brabender Instruments, Inc.; or Brabender OHG


54-21.02, Rheological Behavior of Flour by Farinograph: Constant Flour Weight Procedure

Farinograph. C. W. Brabender Instruments, Inc.; or Brabender OHG


54-22.01, Rheological Behavior of Flour by Farinograph: Constant Dough Weight Procedure

Farinograph. C. W. Brabender Instruments, Inc.; or Brabender OHG


54-30.02, Alveograph Method for Soft and Hard Wheat Flour

Alveograph. Chopin S.A.; U.S. and Canadian distributor: YSI Laboratory Equipment


54-40.02, Mixograph Method

Mixograph and bowl. National Manufacturing Co.;
Timer, Gra-Lab. Fisher Scientific


54-50.01, Determination of the Water Absorption Capacity of Flours and of Physical Properties of Wheat Flour Doughs, Using the Consistograph

Consistograph, including the mixer and the recorder-calculator with printer. Chopin S.A.. U.S. and Canadian distributor: Labsynergy


54-60.01, Determination of Rheological Behavior as a Function of Mixing and Temperature Increase in Wheat Flour and Whole Wheat Meal by Mixolab

Mixolab. CHOPIN Technologies.
Laboratory mill. Model 3100 or 120. PerkinElmer North America, Inc.


54-70.01, High-Speed Mixing Rheology of Wheat Flour Using the doughLAB

doughLAB. part N102182, PerkinElmer North America, Inc.
Mixing chamber/bowl. part N102267 (300 g), PerkinElmer PerkinElmer North America, Inc.
Software for Windows (DLW). part N104987, PerkinElmer, PerkinElmer North America, Inc.


55-10.01, Test Weight per Bushel

Weight per bushel tester. Seedburo Equipment Co.


55-30.01, Particle Size Index for Wheat Hardness

Laboratory mill. Model LM 3303/3310, PerkinElmer North America, Inc.


55-31.01, Single-Kernel Characterization System for Wheat Kernel Texture

Single-kernel characterization system. PerkinElmer North America, Inc.
Sieve shaker. Ro-Tap model, available from Cole-Parmer Instrument Co.; or Fisher (Cat. no. 04 909), Fisher Scientific; or W. S. Tyler Co.


55-40.01, Particle Size of Wheat Flour by Laser Instrument

Microtrac particle analyzer. Microtrac


56-11.02, Solvent Retention Capacity Profile

Centrifuge tubes


56-15.01, Solvent Retention Capacity Profile – Automated Measurement

Centrifuge tubes, 50-ml, polypropylene, conical bottom (VWR Ref. No. 525-0402 is an example of suitable tubes)
Syringes, 30-ml, with central outlet (VWR Ref. No. 5300.X00D0 is an example of suitable syringes)


56-21.01, Flour Swelling Volume

Hot water bath, circulating
Culture tubes
Flour swelling volume racks and tubes


56-25.01, Oxidative Gelation of Aqueous Wheat Flour Suspensions

Bostwick consistometer. CSC Scientific Company, Inc.


End-over-end test tube shaker. Several sources, including: LabQuake shaker, Thermo Fisher Scientific; or Perten Scientific ShakeMatic, PerkinElmer


56-40.01, Water Hydration (Absorption) of Rolled Oats

U.S. no. 20 standard sieve (Cat. no. 04-881-10M) and receiver pan (Cat. no. 04-887B). Fisher Scientific, part of Thermo Fisher Scientific


56-61.02, Sedimentation Test for Wheat

Grinding mill suitable for small samples. Falling Number model 3303. PerkinElmer; or Cyclone, Udy Corp.


56-63.01, Micro Sedimentation Test for Wheat

Grinding mill suitable for small samples. Laboratory Mill model 3303/3310. Perkin Elmer; or Cyclone, Udy Corp.


56-70.01, Sodium Dodecyl Sulfate Sedimentation Test for Durum Wheat

Grinding mill. Cyclone, Udy Corp.


56-81.04, Falling Number Determination

Falling number apparatus. Perkin Elmer
Sample mill. Several sources, including: Mikro sample mill, Hosokawa Micron Powder System; Laboratory Mill model LM 3100 or LM 120, Perkin Elmer; and Udy Cyclone


57-12.01, Method for Determining Water Hydration Capacity and Percentage of Unhydrated Seeds of Pulses

Weighing dishes (Cat. no. 02-202-100). Fisher Scientific
Dredger (Cat. no. 7471). Johnson-Rose Corp.
Tray, stainless steel (Cat. no. 13-361A, or equivalent). Fisher Scientific


57-13.01, Water-Holding Capacity of Pulse Flours and Protein Materials

Centrifuge tube, Cat. no. NC9031845, Fisher Scientific
Culture test tubes, Cat. no.14-961-29, Fisher Scientific
Filter cloth, Miracloth, Cat. no. 475855-1R, Millipore Sigma
Stirring rods, Cat. no.59060-047, VWR
Syringe barrel, Cat. no.CABD301032, VWR
Transfer pipette, Cat. no.13-711-41, Fisher Scientific


58-12.01, Determination of Oil Color

AOCS-Tintometer apparatus or Lovibond tintometer, model AF710. U.S. supplier: HF Scientific, a Watts Brand
Tintometer Color Scale (for AOCS method Cc 13b-45). American Oil Chemists’ Society (AOCS)
Color tubes. Cat. no. 220-260-943, Fisher Scientific or Wilkens-Anderson Co.


58-14.01, Consistency-Penetration Method

Penetrometer and cone. Fisher Scientific; or VWR Scientific Products


58-16.01, Determination of Peroxide Value-Acetic Acid-Chloroform Method

Soluble starch solution. Soluble potato starch for iodometry, Cat. no. 4006-04, Biocompare
soluble starch for iodometry, Cat. no. S516-100, Fisher Scientific
soluble potato starch, Cat. no. S-2630, or Sigma Chemical Co.


58-20.02, Refractive Index

Refractometer. Abbé 60 (standard, high accuracy, or automatic), Jepson Bolton and Co., Ltd.


58-25.01, Solid Fat Index-Dilatometric Method

Dilatometers, Pyrex. Cat. no 329300-0000 and 329325-0000, Kimble Kontes, a DWK Life Sciences Company


58-30.02, Iodine Value of Fats and Oils-Cyclohexane-Acetic Acid Method

Soluble starch solution. Soluble potato starch for iodometry, Cat. no. 4006-04, Biocompare
soluble starch for iodometry, Cat. no. S516-100, Fisher Scientific
soluble potato starch, Cat. no. S-2630, or Sigma Chemical Co.


58-39.01, Dropping Point-Automatic Determination of Melting Point in Fats

Control unit (Mettler FP90), dropping point furnace (Mettler FP83-HT), sample cup (no. 18712), sample cup holder (no. 18711), and sample cup collecting sleeve (no. 18713). Mettler Toledo


58-45.01, Monoglycerides and Free Glycerol

Starch. Several are available, including: soluble starch for iodometry (Fisher Scientific Cat. no. S516-100). Fisher Scientific.
Soluble potato starch (Sigma S 2630). Sigma Chemical Co..
Soluble potato starch for iodometry (Cat. no. 4006-04). Biocompare


58-54.02, Oil Stability Index

Instruments to measure conductivity of liquids. Rancimat, model 743, Metrohm USA, Inc.
Purge gas dryer, Balston model 75-45EU for FT-IR, and trace hydrocarbon remover, model A963-000. Parker, Inc.
Thermometer calibrated against a standard. Cat. no. 3551 RM (temperature range 95–150° in 0.1° finest readable interval, 76-mm immersion), Brooklyn Thermometer; or (for temperature calibration of the Rancimat analyzer), calibrated platinum resistance (RTD) digital thermometer with calibration points at 100, 110, 120, 130 and 140°, Omega Engineering, Inc.
Standard for thermometer National Institute of Standards and Technology (NIST)
Digital flow meter for airflow measurement. Model 7080, Alltech Associates
Reaction tubes, all-glass assembly. Cat. no. EA 1087, Brinkmann Instruments, Inc.
Silicone antifoam. Dow Corning Corp.


61-01.01, Amylograph Method for Milled Rice

Brabender Visco/Amylo/Graph. C. W. Brabender Instruments, Inc.; or Brabender OHG


61-02.01, Determination of the Pasting Properties of Rice with the Rapid Visco Analyser

Rapid Visco Analyser, models RVA-Super 4, RVA-4, or RVA-TecMaster or RVA-Starchmaster2 and obsolete models. Perkin Elmer
Sample mill. Cyclotec model 1093, cyclone fitted with 0.5-mm screen, Foss North America; or Newport model 6200 cereal mill fitted with 0.5-mm screen, Newport Scientific Pty. Ltd.; or Cyclone fitted with 0.5-mm screen, Udy Corp.


61-03.01, Amylose Method for Milled Rice

Cyclone grinder, with a 0.5-mm screen. Udy Corp.; or Foss North America; or Wig-L-Bug Amalgamator, Dentsply, Inc.


61-04.01, Gelatinization Temperature of Milled Rice Flour

Rapid Visco Analyser, PERNS106947 (RVA 4800), PERNS105179 (RVA 4500), PERNS103097 (RVA-TecMaster), Perkin Elmer
Canisters and paddles set, quantity 200, N102774, Perkin Elmer


62-05.01, Preparation of Sample: Bread

Sieve equipment. W. S. Tyler, Inc.


64-71.01, Mechanical Sampling of Dry, Free-Flowing Granular Material

DT and SM sampler systems. For information, regarding use, contact Standards and Procedures Branch, FGIS, GIPSA, USDA


66-20.01, Determination of Granularity of Semolina and Farina: Sieving Method

Sieve shaker. Cole-Parmer, Cole-Parmer Instrument Co.; Fisher (Cat. no. 04 909), Fisher Scientific; or Ro-tap (model RX 29), W. S. Tyler Co.


66-41.01, Macro Scale Pasta Processing

Extruder. Wenger; Namad Impianti
Mixer. Hobart
Pasta dryer Clextral; Buhler Inc.; or Pavan SpA


66-42.01, Micro Scale Pasta Processing

Farinograph and bowl. C. W. Brabender Instruments, Inc.; or Brabender OHG
Pasta dryer. Buhler Inc.; or Pavan SpA


66-50.01, Pasta and Noodle Cooking Quality-Firmness

Texture analyzer. Stevens texture analyzer, Brookfield Engineering Laboratories, Inc.
Instron Universal Testing Machine. Instron Corp.; or TA-XT2 texture analyzer, Texture Technologies Corp.


66-51.01, Fully Cooked Time of Spaghetti

Hotplate. Capable of heating at least 300 mL of water to a rolling boil and provide even heating across its surface; power at least ~1,200 W.
Titanium pot. Approximate dimensions 116 mm height × 104 mm width. Example by Evernew, ultra-light titanium pasta pot, 750 mL. Model: ECA-521R, made in Japan.


66-52.01, Determination of Cooked Spaghetti Firmness

Texture Analyser. Stable Micro Systems Texture Analyser (TA.XT2i, TA.XT Plus, TA.HD Plus, or TA.HDi) equipped with a 5 kg or higher load cell. Texture Analyser software (Exponent, Exceed, Expert).
Blade. For the cooked pasta, use knife blade TA-47 (also called A/LKB-F2 in some markets; dimensions 32 mm length × 55 mm width, tapered blade 1 mm thick) on the metal or Perspex base plate platform (TA-90) from Texture Technologies Corp., Scarsdale, NY USA; users outside the United States and Canada, pasta knife blade A/LKB-F2.
Titanium pot. Approximate dimensions 116 mm height × 104 mm width. Example by Evernew, ultra-light titanium pasta pot, 750 mL. Model: ECA-521R, made in Japan.


66-60.01, Guidelines for Making and Cooking Japanese Udon Noodles

Mixer. model N50, Hobart Corporation,
Noodle machine. Ohtake Noodle Machine Company


70-50.02, Preparation and Standardization of Potassium Permanganate Solution

Sodium oxalate. Standard reference material 40, National Institute of Standards and Technology (NIST)


70-60.01, Preparation and Standardization of Silver Nitrate Solution

Standard reference material. National Institute of Standards and Technology (NIST)


70-70.01, Preparation and Standardization of Sodium or Potassium Hydroxide Solution

Potassium hydrogen phthalate. Standard reference material for acidimetry 84, National Institute of Standards and Technology (NIST)


70-75.01, Preparation and Standardization of Sodium Thiosulfate Solution Potassium dichromate

Standard reference material 136e, National Institute of Standards and Technology (NIST)


74-09.01, Measurement of Bread Firmness by Universal Testing Machine

Testing machine. Universal Testing Machine, Instron Corp. or TAXT2 Texture Analyzer, Texture Technologies Co.


74-10.02, Measurement of Bread Firmness-Compression Test

Baker compressimeter.


76-11.01, Starch-Glucoamylase Method with Subsequent Measurement of Glucose with Glucose Oxidase

Glucoamylase (from Rhizopus delemar). amyloglucosidase Cat. no. 11 20 2332001. Roche Diagnostics; or Dupont Nutrition and Biosciences; or Shin Nihon Chemical Co.
Glucoamylase (from Rhizopus delemar), enzyme-buffer-chromogen mixture. Cat. no. 172426, Millipore Sigma; or Glucostat special Sigma Chemical Co.
Enzymes and reagents. Glucose oxidase (Type II from Aspergillus niger) Cat. no. G 7773; peroxidase (Type I from horseradish) Cat. no. P 8125; o-dianisidine dihydrochloride, cat no. D 3252, Sigma Chemical Co.


76-13.01, Total Starch Assay Procedure (Megazyme amyloglucosidase/αAmylase Method)

Total starch assay kit. Cat. no. K-TSTA, Megazyme International Ireland Ltd.
Micropipetters: Pipetman (100-μl) and motorized dispenser (EDP-2). Rainin Instrument Co.
Positive displacement pipetter. Eppendorf 4830 pipetter, variable volume (Brinkmann no. 22-44-050-1; VWR Cat. no. 53512-230), VWR Scientific Products; Brinkmann Instruments, Inc.
Vortex mixer. Thermolyne Maxi-Mix II, Thermo Fisher Scientific; Cat. no. 58810-185, VWR Scientific Products
MOPS. Sodium salt M 9381, Sigma Chemical Co.


76-21.01, General Pasting Method for Wheat or Rye Flour or Starch Using the Rapid Visco Analyser

Rapid Visco Analyser, models RVA-Super 4, RVA-4, or RVA-TecMaster or RVA-Starchmaster2 and obsolete models. Perkin Elmer


76-22.01, Pasting Properties of Oat-Rapid Viscosity Analysis

Rapid Visco Analyser, models RVA-Super 4, RVA-4, or RVA-TecMaster or RVA-Starchmaster2 and obsolete models. PerkinElmer, Inc.
RVA canisters and paddles. PerkinElmer, Inc.


76-30.0, Damaged Starch

α-Amylase preparation, from Aspergillus oryzae. 150–250 Sigma units/mg protein (Cat. no. A 6211), Sigma Chemical Co.


76-31.01, Determination of Damaged Starch-Spectrophotometric Method

Enzymes for damaged starch assays: fungal α-amylase,from Aspergillus oryzae; amyloglucosidasefrom A. niger; glucose oxidase/peroxidase enzyme system (all three supplied in starch damage kit, Cat. no. K-SDAM). Megazyme International Ireland Ltd.


76-33.01, Damaged Starch -- Amperometric Method by SDmatic

SDmatic. Chopin S.A. U.S. and Canadian distributor: www.labsynergy.comxylem Lab Solutions


80-04.01, Determination of Simple Sugars in Cereal Products-HPLC Method

HPLC system (pump and column). Waters Associates
Chromatography software. Millennium 2010 (version 2.15), Waters Associates
Fructose (Cat. no. F 0127); glucose; sucrose (Cat. no. S 9378); maltose (Cat. no. M 5885); and lactose (Cat. no. L 3625). Sigma Chemical Co.


80-05.01, Determination of Saccharides by Liquid Chromatography

Chromatographic column. Aminex HPX-87C (Cat. no. 125-0094) for separating DP1-DP4 saccharides or Aminex HPX-42C (Cat. no. 125-0096) or HPX-42A (Cat. no. 125-0097) for separating DP1-DP7 saccharides, Bio-Rad Laboratories
Ion exchange resin. Cat. no. TMD-8, Biocompare
Maltotriose. Cat. no. M 8378, Sigma Chemical Co.
Maltose hydrate. HHH, Hayashibara Co. Ltd.


80-10.01, Glucose in Sugar Mixtures-Glucose Oxidase Method

Purified glucose oxidase. Cat. no. 346385, Millipore Sigma


86-02.01, Vitamin A-Carr-Price Method

Carr-Price reagent (antimony trichloride). Cat. no. 311375, Sigma Chemical Co.
Vitamin A reference solution. U.S. Pharmacopeia Convention, Inc.


86-03.01, Vitamin A in Enriched Flour

Carr-Price reagent (antimony trichloride). Cat. no. 311375, Sigma Chemical
Vitamin A reference solution. U.S. Pharmacopeia Convention, Inc.


86-05.01, Vitamin A and Carotene

UV light. UVP, LLC
Carr-Price reagent (antimony trichloride). Cat. no. 311375, Sigma Chemical Co.
Vitamin A reference solution. U.S. Pharmacopeia Convention, Inc.
Carotene reference crystals. Mixed isomers, 1:2 β:α, from carrots, 95% pure, HPLC grade and type IV from carrots, substantially free of α-carotene,  Sigma Chemical Co.
Carotenes. Purified chemicals with which to make a mixture of α- and β-carotenes. Trans-β-Carotene, Cat. no. C9750; β-carotene, 95% pure, Cat. no. 22040; α-carotene, xanthophyll, HPLC grade, 90% pure, Cat. no. 95507, Sigma Chemical Co.; or type I β-carotene, synthetic, Cat. no. C9750; type II β-carotene, HPLC grade, Cat. no. C4582; and α-carotene, xanthophyll, 70% pure, Cat. no. X6250, Sigma Chemical Co.


86-06.01, Analysis of Vitamins A and E by High-Performance Liquid Chromatography

Chromatographic column, for vitamin A, reverse-phase C18, 10-µm. Vydac TP201, Cat. no. 1005, GRACE
Chromatographic column, for vitamin E, reverse-phase C8, 10-µm. Lichrosorb RSIL C8, GRACE; or Sigma Chemical Co.
Certified USP vitamin A. U.S. Pharmacopeia Convention, Inc.
Vitamin E acetate. Cat. no. 248177, Sigma Chemical Co.


86-10.01, Ascorbic Acid-Indophenol-Xylene Extraction Method

Ascorbic acid standard. Cat. no. 1043003, United States Pharmacopeia Convention, Inc.


86-31.01, Vitamin B6 Complex-Microbiological Method

Yeast, Saccharomyces uvarum. Cat. no. 76514, American Type Culture Collection [ATCC]
Casein hydrolysate solution Cat. no. DF-0288-15 or DF-0288-17, Fisher Scientific


86-40.01, Vitamin B12-Microbiological Method

Stock culture, of Lactobacillus delbrueckii ssp. lactis. Cat. no. 10697, American Type Culture Collection [ATCC]
Standard cyanocobalamin stock solution. Cat. no. 15200, United States Pharmacopeia Convention, Inc.


86-45.01, Choline

Choline chloride. Cat. no. 239941, Sigma Chemical Co.


86-47.01, Total Folate in Cereal Products-Microbiological Assay Using Trienzyme Extractions

Centrifuge, clinical, to accommodate 20-× 150-mm test tubes.
Spectrophotometer to read 20-× 150- (or 18-× 150-mm) test tubes.
Covered water batch, 37 ± 0.2°, with rotary shaker.
Disposable glass test tubes, borosilicate glass, 20-× 150-mm (or 18-× 150-mm), Cat. no. T1290-10A or T1290-9A; pH meter, with long combination electrode, Orion SA720, Cat. no. 072000; Vortex mixer, Cat. no. S8223-1; disposable tips, Eppendorf 1.25-ml, Cat. no. P5063-28.
Pipetting machine(s), to deliver 1- and 5-ml aliquots. Vial filler, Filamatic
Autoclave, for sterilizing at 15 psi and 121-123°. No. N67CDS, Allegiance Healthcare, Div. Of Cardinal Health
Centrifuge tubes, disposable. Falcon Plastics, Inc
Balance, analytical, reading to at least four places Fisher Scientific
Balance, top loading, reading to three places, Fisher Scientific
Distilling apparatus for water. No. A1212, Thermo Fisher Scientific
Sample change, automated, assay-tube reading, modified with an air agitation system and connected to a spectrophotometer with a flow cell and either a printer or a computer. Escargot fractionator, model SC-30 or model 222, Gilson Co.
Sterilization Filter units, 0.22-µm (15-ml, 500-ml, and 1-liter size). Nalge disposable Filterware, Thermo Fisher Scientific, International Dept.; or disposable filterware, Corning Glass Works
Agar. Bacto, Cat. no. DF0140-01, Difco Laboratories
Agar, pure culture of Lactobacilli casei. Cat. no. 7469, American Type Culture Collection [ATCC],; or Cat. no. DF090015, Difco Laboratories
α-Amylase. No A-6211, Sigma Chemical Co.
Antifoam. Antifoam AF or B, Dow Corning Corp.
Casein hydrolysate, vitamin-free acid-hydrolyzed. Hy case amino, ICN Conjugase source. Chicken pancreas, desiccated. Cat. no. DF0459122, Labsource
Folic acid reference standard. Cat. no. 28600, U.S. Pharmacopeia Convention, Inc.
Liver. Cat. no. DF2133320, Labsource
Potassium phosphate, monobasic.
Protease, pronase E. Cat. no. P5147, Sigma Chemical Co.
Folic acid casei medium. Cat. no. 90004040, Difco Laboratories
Bacto micro inoculum broth. Cat. no. 0320-02, Difco Laboratories
Data anlysis software for 96-well microplate method. Microplate Manager, Bio-Rad Laboratories


86-49.01, Niacin in Enrichment Concentrates

Nicotinic acid standard. VWR Scientific Products


86-50.02, Niacin and Niacinamide in Cereal Products

Nicotinic acid standard. VWR Scientific Products


86-51.01, Niacin-Microbiological Method

Activated charcoal (Darco G60) and nicotinic acid standard. VWR Scientific Products
Stock culture, of Lactobacillus plantarum. Cat. no. 14917, American Type Culture Collection [ATCC]
Bacto-niacin assay medium. Difco Cat. no. DF0322, Fisher Scientific


86-70.01, Riboflavin-Fluorometric Method

Dry U.S.P. riboflavin reference standard. Cat. no. 603006, United States Pharmacopeia Convention, Inc.


86-72.01, Riboflavin-Microbiological Method

Dry U.S.P. riboflavin reference standard. Cat. no. 603006, United States Pharmacopeia Convention, Inc.
Lactobacillus casei ssp. rhamnosis. Cat. no. 11981, American Type Culture Collection [ATCC]


86-80.01, Thiamine-Thiochrome Method

Absorption tubes. Chromatographic tubes, Cat. no. 12-570-200, Fisher Scientific
Thiamine hydrochloride. Cat. no. 65600-2, United States Pharmacopeia Convention, Inc.
Base-exchange silicate, Bio-Rex 70, 50-100 mesh, sodium form. Cat. no. 142-5832, Bio-Rad Laboratories
Base-exchange silicate. Decalso, Sargeant-Welch Scientific Co.


86-90.01, B-Vitamins in Vitamin Concentrations by HPLC

Detector. Waters model 486 tunable UV/visible absorbance detector (also, replacement parts for model 440 absorbance detector), Waters Associates; or Varichrom variable-wavelength absorbance detector (model 9050). Varian Inc. Scientific Instruments
Pump. Beckman model 110B or 118, Beckman Instruments, Inc.
Column. Lichrosorb RP-8, 10 µm, 250-× 4.6-mm, Cat. no. 8843, Grace, or µ-Bondapak-C18, Waters Associates


89-01.01, Yeast Activity, Gas Production

Pressuremeter. National Manufacturing
Rheofermentometer. Chopin S.A.; U.S. and Canadian distributor: PerkinElmer, Inc.,
Risograph.
SJA fermentograph. Mekab Machine