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Low α-Amylase Starch Digestibility of Cooked Sorghum Flours and the Effect of Protein1

September 1998 Volume 75 Number 5
Pages 710 — 713
Genyi Zhang 2 and Bruce R. Hamaker 2 , 3

Paper No. 15505 from the Purdue Agricultural Experiment Station. Department of Food Science and the Whistler Center for Carbohydrate Research, Purdue University, 1160 Smith Hall, West Lafayette, IN 47907-1160. Corresponding author. E-mail: hamakerb@foodsci.purdue.edu


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Accepted June 12, 1998.
ABSTRACT

The comparably low starch digestibility of cooked sorghum flours was studied with reference to normal maize. Four sorghum cultivars that represent different types of endosperm were used. Starch digestibilities of 4% cooked sorghum flour suspensions, measured as reducing sugars liberated following α-amylase digestion, were 15–25% lower than for cooked maize flour, but there were no differences among the cooked pure starches. After the flours were predigested with pepsin to remove some proteins, the starch digestibility of cooked sorghum flours increased 7–14%, while there was only 2% increase in normal maize; however, there was no effect of pepsin treatment on starch digestibility if the flours were first cooked and then digested. After cooking with reducing agent, 100 mM sodium metabisulfite, starch digestibility of sorghum flours increased significantly while no significant effect was observed for maize. Also, starch solubility of sorghum flours at 85 and 100°C was lower than in maize, and sodium metabisulfite increased solubility much more in sorghum than in maize. Differential scanning calorimetry results of the flour residue after α-amylase digestion did not show any peaks over a temperature range of 20–120°C, indicating that sorghum starches had all undergone gelatinization. These findings indicate that the protein in cooked sorghum flour pastes plays an important role in making a slowly digesting starch.



© 1998 American Association of Cereal Chemists, Inc.