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Development of a Germination Process for Producing High β-Glucan, Whole Grain Food Ingredients from Oat

November 2001 Volume 78 Number 6
Pages 715 — 720
A. Wilhelmson , 1 , 2 K.-M. Oksman-Caldentey , 1 A. Laitila , 1 T. Suortti , 1 A. Kaukovirta-Norja , 1 and K. Poutanen 1

VTT Biotechnology, P.O. Box 1500, FIN-02044 VTT (Espoo), Finland. Corresponding author. Phone: +358 9 456 7113. Fax +358 9 455 2103. E-mail: annika.wilhelmson@vtt.fi


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Accepted August 14, 2001.
ABSTRACT

Germination can be used to improve the texture and flavor of cereals. However, germination generally causes breakdown of β-glucans, which is undesirable with respect to the functional properties of β-glucan. Our aim was to assess possibilities of germinating oat without substantial loss of high molecular weight β-glucan. Two cultivars, hulled Veli and hull-less (naked) Lisbeth were germinated at 5, 15, and 25°C and dried by lyophilization or oven drying. Elevated germination temperatures led to an increase in Fusarium, aerobic heterotrophic bacteria, Pseudomonas spp., lactic acid bacteria, enterobacteria, and aerobic spore-forming bacteria. Therefore, the germination temperature should be kept low to avoid excessive growth of microbes. Of the samples germinated at 15°C, only one contained low amounts of the Fusarium toxin deoxynivalenol (52 μg/kg). Germination led to the breakdown of β-glucans, but the decrease in the molecular weight of β-glucan was initially very slow. A short germination schedule (72 hr, 15°C) terminated with oven drying was developed to produce germinated oat with retained β-glucan content. Compared with the native oat, 55–60% of the β-glucan could be retained.



© 2001 American Association of Cereal Chemists, Inc.