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A Fast Colorimetric Method to Estimate Corn Quality Loss

July 2003 Volume 80 Number 4
Pages 450 — 453
E. A. Tosi 1 , 2 and E. D. Ré 1

Centro de Investigación y Desarrollo en Tecnología de Alimentos, Facultad Regional Rosario, Universidad Tecnológica Nacional, Zeballos 1341, S2000 BQA, Rosario, Argentina. Corresponding author. E-mail: tosienzo@ciudad.com.ar. Telefax: 54(0)341-4484909.


Go to Article:
http://dx.doi.org/10.1094/CCHEM.2003.80.4.450
Accepted June 24. 2002.
ABSTRACT

A fast method to estimate the loss of corn quality for wet milling as a result of excessive thermal treatment during drying is proposed. Proteins that remain soluble after drying were quantitatively extracted with a solution of phosphoric acid and ethanol. The soluble proteins were colorimetrically quantified using Coomasie Brillant Blue G dye and measuring at 595 nm. A decrease in absorbance values indicates a reduction in commercial quality of the corn. The application time of the proposed technique is ≈30 min.


© 2003 American Association of Cereal Chemists, Inc.