November
2003
Volume
80
Number
6
Pages
755
—
763
Authors
N. M.
Edwards
,
1
,
2
S. J.
Mulvaney
,
3
M. G.
Scanlon
,
4
and
J. E.
Dexter
1
Affiliations
Canadian Grain Commission, 1404-303 Main St., Winnipeg, MB, R3C 3G8, Canada. GRL contribution no. 847.
Corresponding author. Phone: 204-983-8033. Fax: 204-983-0724. E-mail: nedwards@grainscanada.gc.ca.
Dept. Food Sci. Cornell University, Stocking Hall, Ithaca, NY.
Dept. Food Sci., University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
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RelatedArticle
Accepted May 15, 2003.
Abstract
ABSTRACT
Gluten was isolated from three durum wheat cultivars with a range in strength. Gluten was further fractionated to yield gliadin, glutenin and high molecular weight (HMW) and low molecular weight (LMW) glutenin subunits (GS). The gluten and various fractions were used to enrich a base semolina. Enriched dough samples were prepared at a fixed protein content using a 2-g micromixograph. Mixing strength increased with addition of gluten. Dynamic and creep compliance responses of doughs enriched with added gluten ranked in order according to the strength of the gluten source. Gliadin addition to dough resulted in weaker mixing curves. Gliadin was unable to form a network structure, having essentially no effect on dough compliance, but it did demonstrate its contribution to the viscous nature of dough (increased tan δ). Source of the gliadin made no difference in response of moduli or compliance. Addition of glutenin to the base semolina increased the overall dough strength properties. Glutenin source did influence both dynamic and compliance results, indicating there were qualitative differences in glutenin among the three cultivars. Enrichment with both HMW-GS and LMW-GS increased overall dough strength. Source of HMW-GS did not affect compliance results; source of LMW-GS, however, did have an effect. The LMW-2 proteins strengthened dough to a greater extent than did LMW-1. Mechanisms responsible for dough viscoelastic properties are described in terms of reversible physical cross-links.
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ArticleCopyright
This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. American Association of Cereal Chemists, Inc., 2003.