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Increase in Apparent Amylose Content and Change in Starch Pasting Properties at Cool Growth Temperatures in Mutant Wheat

¹National Institute of Crop Science (NICS), 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan. ²Present address of corresponding author: National Agricultural Research Center for Western Region (WeNarc); 1-3-1 Senyu, Zentsuji, Kagawa 765-8508, Japan. Fax: +81-877-63-1683. E-mail: tyanagi@affrc.go.jp

Some mutant wheat lines with low-amylose content were grown in a field and greenhouse (15 or 20°C) to compare apparent amylose content and starch pasting properties. The apparent amylose content of flour and starch increased and starch pasting parameters as measured by a Rapid Visco Analyser (RVA) changed in the greenhouse (at cool temperatures) during seed maturation. Densitometric analysis of the protein band separated by electrophoresis suggested that the increase in amylose content by cool temperature was related to the amount of Wx-D1 protein. This data suggests that the Wx-D1 gene was responsible for these changes. In wheat starch from Tanikei A6099 and Tanikei A6598 at 15°C, the value of final viscosity and total setback was higher than that from the field. In wheat starch from Tanikei A6599-4 (waxy mutant with stable hot paste viscosity), the peak viscosity temperature was higher and time maintained >80% of the peak was shorter at 15°C than that from the field. Genetic analysis using doubled-haploid (DH) lines from a combination of Tanikei A6599-4 and Kanto 118 (low-amylose line) showed that apparent amylose content increased and the starch pasting curve and properties changed in waxy progenies similar to Tanikei A6599-4.