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Potential Antioxidant Activity of γ-Oryzanol in Rice Bran as Determined Using an In Vitro Mouse Lymph Axillary Endothelial Cell Model

¹Department of Food Science, Louisiana Agricultural Experimental Station, Louisiana State University Agricultural Center, Baton Rouge, LA 70803. ²Department of Food Science, National Pingtung University of Science and Technology, 1, Shuefu Road, Neipu, Pingtung 91201, Taiwan. ³Corresponding author. Phone: 225-578-5192. Fax: 225-578-5300. E-mail address: SGodber@agcenter.lsu.edu

A mouse lymphatic endothelial cell (SVEC4-10) in vitro model was developed and found to be effective in the study of antioxidant activity of γ-oryzanol in rice bran. The critical and vital parameters in developing these cell models included the emulsion preparation of hydrophobic compounds, the consistent management of cell culture, and the selection of cell viability detection methods compatible with the cell lines and the test substances. The SVEC4-10 cell line had a fast metabolism and consequently could be used to determine antioxidant activity of a test substance in a relatively rapid manner. Results showed that γ-oryzanol, ferulic acid, and α-tocopherol could interact with cells such that oxidative damage induced by tert-butyl hydroperoxide on cellular mitochondrial activity lessened, and in some situations, γ-oryzanol was a more effective antioxidant than α-tocopherol. The results also suggested different antioxidant mechanisms among γ-oryzanol, ferulic acid, and α-tocopherol. The three major components of γ-oryzanol generally had higher antioxidant activity than γ-oryzanol, with 24-methylene cycloartanyl ferulate relatively more effective. Synergistic antioxidant activity among γ-oryzanol, ferulic acid, and α-tocopherol was also found.