November
2012
Volume
89
Number
6
Pages
276
—
283
Authors
Geun-Pyo Hong,1
Nicole Avramenko,1
Andrea Stone,1
Dawn Abbott,2
Hank Classen,2 and
Michael Nickerson1,3
Affiliations
Department of Food and Bioproduct Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, S7N 5A8 Canada.
Department of Animal and Poultry Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, S7N 5A8 Canada.
Corresponding author. Phone: (306) 966-5030. Fax: (306) 966-8898. E-mail: Michael.Nickerson@usask.ca
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RelatedArticle
Accepted July 16, 2012.
Abstract
ABSTRACT
Extractability and molecular modifications of gliadin and glutenin proteins withdrawn from different stages of a commercial ethanol fuel/distillers dried grains with solubles (DDGS) process using a wheat feedstock were investigated. Materials were taken postliquefaction (PL), postdistillation (whole stillage), and postdrying (DDGS) during the process and then fractionated to separate the gliadins and the soluble high- and low-molecular-weight glutenins following a modified Verbruggen extraction method. Each fraction was characterized based on the extraction efficiencies within various aqueous alcohols of propan-1-ol, electrophoretic patterns, intrinsic and extrinsic fluorescence, free and total sulfhydryl content, and total disulfide bond levels. Findings indicated significant changes to the composition of extracted proteins and modifications to the protein structure (i.e., surface properties and conformation) throughout the ethanol/DDGS process, beginning with the first step of production (PL, ≈83°C). Overall, processing resulted in a shift toward an unextractable gluten matrix, accompanied by increases in hydrophobicity, disulfide bridging, and excessive protein aggregation.
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© 2012 AACC International, Inc.