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Isolation of an Antifungal Pathogenesis-Related Protein from Naked Oat (Avena nuda) Seeds

^†Corresponding author. Phone: +86-0351-7018268. E-mail: yaweishi@sxu.edu.cn
Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, China.

A novel antifungal protein was isolated from naked oat (Avena nuda) seeds by acetone fractionation, (NH₄)₂SO₄ precipitation, Q-Sepharose ion-exchange chromatography, chitin affinity chromatography, and Sephacryl S-200 gel filtration chromatography. The antifungal protein exhibited a molecular mass of 23,760, as measured by gel filtration and SDS-PAGE. Matrix-assisted laser desorption ionization–time of flight MS analysis indicated that the protein was homologous with a permatin precursor from A. sativa. The protein from naked oats exhibited antifungal activity against Trametes sp. SQ01 (half-maximal inhibitory concentration [IC₅₀] = 5.68μM), Trichoderma spp. (IC₅₀ = 0.83μM), and Chaetomium sp. R01.