St. Paul, Minn. (March 8, 2010) – The AACC International Approved Methods Technical Committee has released a new method, Quantification of MON 810 Corn in Corn Flour by Real-Time Polymerase Chain Reaction (10-30.01).
AACC Intl. Method 11-30.01 quantifies the relative amount of MON 810 DNA in corn flour. It makes a relative quantitation of a specific part from the taxon-specific maize gene (i.e., the high mobility group [HMG] protein gene) and of the single-copy DNA integration-border region of the genomic sequence and the inserted sequence element. Real-time polymerase chain reaction (PCR) in a TaqMan assay is used. The method is calibrated to estimate the copy number percent of modified corn in corn flour by using a reference material certified for its MON 810 mass fraction. Copy number percents are estimated on the basis of the DNA extracted from the reference material and an assumed average genome size. The method is suitable for enforcement purposes with respect to its trueness and interlaboratory variability.
AACC International Approved Methods of Analysis, 11th Edition online subscribers will receive free access to this new method. To request a subscription quote, go to the Approved Methods of Analysis, 11th Edition website. This method may also be purchased as an individual PDF file for $200. To purchase this single method call toll-free 1.800.328.7560 U.S.A. and Canada or +1.651.454.0766 elsewhere.